... Continue to: https://youtube.com/shorts/wQlsY_6QDdI?si=HynpUKDV_Isa3dSu
A #lactate curve is shaped by the tension between #anaerobic and #aerobic capacity. Judging a curve by only the latter is incomplete and error prone.
I do not #run, but have been meaning for years to add some #anaerobic activity in my life...
A colleague of mine shared about his latest #running achievement (10Km).
I was tempted to share how he inspired me and to commit myself - with my team as witnesses (common psychologic trick)..
However, I decided to keep quiet - and to rely on pure #selfMotivation to drive myself to try running a bit.
My #selfDiscipline succeeded and this week I managed to #run twice - nearly a mile each time!
Gj me!
"In a hot spring at Yellowstone National Park, a microbe does something that life shouldnβt be able to do: It breathes oxygen and sulfur at the same time."
https://www.quantamagazine.org/the-cells-that-breathe-two-ways-20250723/
To advance the utilization of microbial cell factories in gas fermentation processes, their physiological and biotechnological characteristics must be understood. Here, we report on the construction and operation of a novel device, the Gas and Pressure Controller (GPC), which is specifically designed for the automated control of the headspace gas pressure of closed cultivation bottles and facilitates automated gassing, sparging, monitoring and regulation of the headspace volume operated in closed batch cultivation mode in real time.As proof of concept, the physiological and biotechnological characteristics of four autotrophic, hydrogenotrophic methanogenic archaea were examined to quantify novel physiological limits through the elimination of gas limitation during growth and methane formation. We determined unprecedented high maximum specific methane productivity (qCH4) values for: Methanothermobacter marburgensis of 169.59βΒ±β12.52 mmol gββ1 hββ1, Methanotorris igneus of 420.21βΒ±β89.46 mmol gββ1 hββ1, Methanocaldococcus jannaschii of 364.52βΒ±β25.50 mmol gββ1 hββ1 and Methanocaldococcus villosus of 356.38βΒ±β20.79 mmol gββ1 hββ1. Obtained qCH4 of M. marburgensis is more than 10-fold higher compared to conventional closed batch cultivation set-ups and as high as the highest reported qCH4 value of M. marburgensis from fed-batch gas fermentation in stirred tank bioreactors. Furthermore, the GPC demonstrated reliable functionality with Methanococcus maripaludis, operating safely and autonomous during long term cultivation. This novel device enables optimal headspace pressure control, providing flexibility in application for various gas-fermenting biotechnological processes. It facilitates near optimal cultivation conditions in semi-continuous closed batch cultivation mode, the analysis of limiting factors in high-throughput experimental design and allows for automated biomass production of autotrophic, hydrogenotrophic methanogens. Graphical abstract
To advance the utilization of microbial cell factories in gas fermentation processes, their physiological and biotechnological characteristics must be understood. Here, we report on the construction and operation of a novel device, the Gas and Pressure Controller (GPC), which is specifically designed for the automated control of the headspace gas pressure of closed cultivation bottles and facilitates automated gassing, sparging, monitoring and regulation of the headspace volume operated in closed batch cultivation mode in real time.As proof of concept, the physiological and biotechnological characteristics of four autotrophic, hydrogenotrophic methanogenic archaea were examined to quantify novel physiological limits through the elimination of gas limitation during growth and methane formation. We determined unprecedented high maximum specific methane productivity (qCH4) values for: Methanothermobacter marburgensis of 169.59βΒ±β12.52 mmol gββ1 hββ1, Methanotorris igneus of 420.21βΒ±β89.46 mmol gββ1 hββ1, Methanocaldococcus jannaschii of 364.52βΒ±β25.50 mmol gββ1 hββ1 and Methanocaldococcus villosus of 356.38βΒ±β20.79 mmol gββ1 hββ1. Obtained qCH4 of M. marburgensis is more than 10-fold higher compared to conventional closed batch cultivation set-ups and as high as the highest reported qCH4 value of M. marburgensis from fed-batch gas fermentation in stirred tank bioreactors. Furthermore, the GPC demonstrated reliable functionality with Methanococcus maripaludis, operating safely and autonomous during long term cultivation. This novel device enables optimal headspace pressure control, providing flexibility in application for various gas-fermenting biotechnological processes. It facilitates near optimal cultivation conditions in semi-continuous closed batch cultivation mode, the analysis of limiting factors in high-throughput experimental design and allows for automated biomass production of autotrophic, hydrogenotrophic methanogens. Graphical abstract
Simultaneous #aerobic and #anaerobic #respiration in a Yellowstone #thermophile challenges scientific norms
#mightymicrobes #extremophiles #chemolithotroph #genomic #metabolism #bacteria #archea #funded_by_NASA #funded_by_us
https://phys.org/news/2025-03-simultaneous-aerobic-anaerobic-respiration-yellowstone.html
Montana State University has long been a hub for research on the many unique features of nearby Yellowstone National Park, and now a doctoral student in one of the university's microbiology laboratories has published a paper on how some hot-spring-dwelling organisms thrive in their extreme environments.
Scientists reveal new findings on the #ancient_life found in #Yellowstone #hot_springs
#hyperthermophiles #respiration #anaerobic #sulfides #evolution #genomis
https://phys.org/news/2025-02-scientists-reveal-ancient-life-yellowstone.html
In the journal Nature Communications, Montana State University scientists in the College of Agriculture highlight fresh knowledge of how ancient microorganisms adapted from a low-oxygen prehistoric environment to the one that exists today. The work builds on more than two decades of scientific research in Yellowstone National Park by MSU professor Bill Inskeep.