BioResire9h ago
Chromatography Quick Lab Guide by BioResire!
Separate biomolecules via stationary/mobile phases in paper, TLC, column, HPLC.
Internships open: info@bioresire.in #Chromatography #HPLC #TLC #Biochemistry #MolecularBiology #LabTechniques #Biotechnology #BioResire #AnalyticalChemistry #LabEquipment #Science #Chemistry #Research #Biomolecules #SeparationScience #Pharma #Forensics #LabGuide #LifeSciences #BiotechInternship
BioResireFeb 1
🧪 Chromatography simplifies separation 🔬, helping students analyze biomolecules accurately using stationary and mobile phases in modern laboratories 📊.
#Chromatography #BiochemistryLab #AnalyticalTechniques #LifeScienceStudents #HPLC #TLC #Biotechnology #PharmaLabs #LaboratoryMethods #BioResire
13Dec 31
Measuring Caffeine Content At Home https://hackaday.com/2025/12/31/measuring-caffeine-content-at-home/
#Chemistryhacks #Caffeine #Chromatography #Concentration #Experiment #Solvent #Thinlayerchromatography
Measuring Caffeine Content At Home

By far, the most widely used psychoactive substance in the world is caffeine. It’s farmed around the world in virtually every place that it has cropped up, most commonly on coffee plants, tea…

Hackaday
Stephan HackerNov 23
Interesting paper by a team around Takahiro Takayama in Analytical Chemistry. A systematic study of the effect of deuteration on retention time and an optimization of stationary and liquid phases to reduce the effect for better quantitative analysis. doi.org/10.1021/acs.... #Chromatography #LCMS

Mechanistic Study of the Deute...
Mechanistic Study of the Deuterium Effect in Chromatographic Separation for Chemical-Tagging Metabolomics and Its Application to Biomarker Discovery in Metabolic Dysfunction-Associated Steatohepatitis

Over the past decade, numerous metabolomics techniques have been developed using liquid chromatography–mass spectrometry (LC-MS). These methodologies have yielded significant findings and facilitated the identification of biomarkers. Among these, chemical-tagging methodologies combined with isotope surrogate tags have garnered considerable attention as a leading approach. Chemical-tagging reduces labor and costs by eliminating the need for internal standard preparation. However, the chromatographic deuterium effect (CDE) has persisted as a significant challenge. CDE poses a risk of data misinterpretation in metabolomics due to potential differences in matrix effects. Although the CDE mechanism has been partially elucidated, it has primarily been attributed to differences in hydrophobicity. A detailed understanding of CDE mechanisms would be valuable for designing chemical tags and optimizing liquid chromatography (LC) conditions. Moreover, emphasizing the CDE could aid in the separation and purification of deuterated compounds. In this study, we investigated the mechanistic basis of the CDE. Initially, four chromatography columns with different separation modes─octadecyl, octadecyl with a positively charged surface, biphenyl, and pentafluorophenyl (PFP) groups─were evaluated based on retention differences between 1H- and 2H6-labeled chemically tagged metabolites. Among these, the PFP column demonstrated the most effective reduction of the CDE, suggesting that electronic interactions with fluorine stabilized 2H-labeled metabolites. Further optimization using the PFP column showed its efficacy in reducing the level of CDE in human serum samples. Finally, the optimized approach was successfully applied to global metabolomics analysis of serum from a mouse model of metabolic dysfunction-associated steatohepatitis.

ACS Publications
Stephan HackerNov 23
Interesting paper by a team around Takahiro Takayama in Analytical Chemistry. A systematic study of the effect of deuteration on retention time and an optimization of stationary and liquid phases to reduce the effect for better quantitative analysis. https://doi.org/10.1021/acs.analchem.5c00289
#Chromatography #LCMS
Mechanistic Study of the Deuterium Effect in Chromatographic Separation for Chemical-Tagging Metabolomics and Its Application to Biomarker Discovery in Metabolic Dysfunction-Associated Steatohepatitis

Over the past decade, numerous metabolomics techniques have been developed using liquid chromatography–mass spectrometry (LC-MS). These methodologies have yielded significant findings and facilitated the identification of biomarkers. Among these, chemical-tagging methodologies combined with isotope surrogate tags have garnered considerable attention as a leading approach. Chemical-tagging reduces labor and costs by eliminating the need for internal standard preparation. However, the chromatographic deuterium effect (CDE) has persisted as a significant challenge. CDE poses a risk of data misinterpretation in metabolomics due to potential differences in matrix effects. Although the CDE mechanism has been partially elucidated, it has primarily been attributed to differences in hydrophobicity. A detailed understanding of CDE mechanisms would be valuable for designing chemical tags and optimizing liquid chromatography (LC) conditions. Moreover, emphasizing the CDE could aid in the separation and purification of deuterated compounds. In this study, we investigated the mechanistic basis of the CDE. Initially, four chromatography columns with different separation modes─octadecyl, octadecyl with a positively charged surface, biphenyl, and pentafluorophenyl (PFP) groups─were evaluated based on retention differences between 1H- and 2H6-labeled chemically tagged metabolites. Among these, the PFP column demonstrated the most effective reduction of the CDE, suggesting that electronic interactions with fluorine stabilized 2H-labeled metabolites. Further optimization using the PFP column showed its efficacy in reducing the level of CDE in human serum samples. Finally, the optimized approach was successfully applied to global metabolomics analysis of serum from a mouse model of metabolic dysfunction-associated steatohepatitis.

ACS Publications
TogaOct 29

A wee #SwatchWednesday this week.

Now that my Inkvent has arrived (no spoilers!), I've decided to dig out my #Chromatography strips to get a more accurate idea of how they're made up compared to my usual paper towel pseudo-chromatography. You can see how limited the latter is compared to the chromatograms.

The inks here are Tono & Lims Rainy Winter & Space Sunset. They're both chromashading inks with some similar notes but very different end results.

#FountainPenInks

Bose-Einstein-KondensatJul 23, 2025

#2LabsToGoEco: #OpenSource combination of an #analytical (high-performance thin-layer #chromatography) & #bioassay #laboratory

-#solar-powered
-#autosampler, #nebulizer, #incubator, #imager, ...

Paper: https://doi.org/10.1016/j.aca.2025.344103
GitHub: https://github.com/OfficeChromatography/2LabsToGo-Eco
#DIYbio #DIYchem #analytics #lab #instruments #TLC #imaging #PiCam #sustainable

Bose-Einstein-KondensatJun 28, 2025

Development of a low-cost, versatile, #Arduino-controlled #OpenSource #gas #chromatography system for #teaching #analytical #chemistry:

- cost: < US $100
- #Python #GUI

https://doi.org/10.1021/acs.jchemed.1c00868
#DIYbio #DIYchem #lab #instruments #education #STEM #MINT #school #EDU #GC

Ramon van BelzenJun 5, 2025
Agilent has released the 8850 GC #chromatography #chemistry agilent.com/en/product/gas-chr…
Die Forschende Hochschule HofJun 5, 2025

🧪 Now we’re talking – Episode 5:
Meet the gel permeation chromatograph! This machine helps us understand how big (or small) molecules really are 🧬 — and plays a key role in basic research at Hof University of Applied Sciences.

🔗 https://t1p.de/dz3ut

#dieforschendehochschulehof #hierwirdknallhartgeforscht #ScienceMastodon #LabTalk #MolecularScience #Chromatography #ResearchTools #AppliedScience