Ramon van Belzenfor GC-MS spectra intrepetation, whats better? Claude Opus or ChatGPT 5.5? #gcms #spectromist #massspec #analyticalchemistry #chromatography ahy
Idiot BusinessSelling pre-owned chromatographs for pets! Because your hamster a nuanced analysis of its diet's organic compounds. #chromatography #hamsterlife #sciencepets
#BusinessIdea #Business #Ai #LLM
#BusinessIdea #Business #Ai #LLM
Labmate Scientific LLCGas Chromatography for Molecular and Volatility Profiling
Labmate Gas Chromatography systems offer precise temperature control and flexible sample introduction for accurate separations. They support identification and quantification of volatile and semi-volatile compounds. Suitable for environmental, petrochemical, pharmaceutical, and food testing workflows.
BioResireChromatography Quick Lab Guide by BioResire!
Separate biomolecules via stationary/mobile phases in paper, TLC, column, HPLC.
Internships open: [email protected] #Chromatography #HPLC #TLC #Biochemistry #MolecularBiology #LabTechniques #Biotechnology #BioResire #AnalyticalChemistry #LabEquipment #Science #Chemistry #Research #Biomolecules #SeparationScience #Pharma #Forensics #LabGuide #LifeSciences #BiotechInternship
Separate biomolecules via stationary/mobile phases in paper, TLC, column, HPLC.
Internships open: [email protected] #Chromatography #HPLC #TLC #Biochemistry #MolecularBiology #LabTechniques #Biotechnology #BioResire #AnalyticalChemistry #LabEquipment #Science #Chemistry #Research #Biomolecules #SeparationScience #Pharma #Forensics #LabGuide #LifeSciences #BiotechInternship
🧪 Chromatography simplifies separation 🔬, helping students analyze biomolecules accurately using stationary and mobile phases in modern laboratories 📊.
#Chromatography #BiochemistryLab #AnalyticalTechniques #LifeScienceStudents #HPLC #TLC #Biotechnology #PharmaLabs #LaboratoryMethods #BioResire
#Chromatography #BiochemistryLab #AnalyticalTechniques #LifeScienceStudents #HPLC #TLC #Biotechnology #PharmaLabs #LaboratoryMethods #BioResire
13Measuring Caffeine Content At Home https://hackaday.com/2025/12/31/measuring-caffeine-content-at-home/
#Chemistryhacks #Caffeine #Chromatography #Concentration #Experiment #Solvent #Thinlayerchromatography
#Chemistryhacks #Caffeine #Chromatography #Concentration #Experiment #Solvent #Thinlayerchromatography
Stephan HackerInteresting paper by a team around Takahiro Takayama in Analytical Chemistry. A systematic study of the effect of deuteration on retention time and an optimization of stationary and liquid phases to reduce the effect for better quantitative analysis. doi.org/10.1021/acs....
#Chromatography #LCMS
Mechanistic Study of the Deute...
Mechanistic Study of the Deute...
Mechanistic Study of the Deuterium Effect in Chromatographic Separation for Chemical-Tagging Metabolomics and Its Application to Biomarker Discovery in Metabolic Dysfunction-Associated Steatohepatitis
Over the past decade, numerous metabolomics techniques have been developed using liquid chromatography–mass spectrometry (LC-MS). These methodologies have yielded significant findings and facilitated the identification of biomarkers. Among these, chemical-tagging methodologies combined with isotope surrogate tags have garnered considerable attention as a leading approach. Chemical-tagging reduces labor and costs by eliminating the need for internal standard preparation. However, the chromatographic deuterium effect (CDE) has persisted as a significant challenge. CDE poses a risk of data misinterpretation in metabolomics due to potential differences in matrix effects. Although the CDE mechanism has been partially elucidated, it has primarily been attributed to differences in hydrophobicity. A detailed understanding of CDE mechanisms would be valuable for designing chemical tags and optimizing liquid chromatography (LC) conditions. Moreover, emphasizing the CDE could aid in the separation and purification of deuterated compounds. In this study, we investigated the mechanistic basis of the CDE. Initially, four chromatography columns with different separation modes─octadecyl, octadecyl with a positively charged surface, biphenyl, and pentafluorophenyl (PFP) groups─were evaluated based on retention differences between 1H- and 2H6-labeled chemically tagged metabolites. Among these, the PFP column demonstrated the most effective reduction of the CDE, suggesting that electronic interactions with fluorine stabilized 2H-labeled metabolites. Further optimization using the PFP column showed its efficacy in reducing the level of CDE in human serum samples. Finally, the optimized approach was successfully applied to global metabolomics analysis of serum from a mouse model of metabolic dysfunction-associated steatohepatitis.
Stephan HackerInteresting paper by a team around Takahiro Takayama in Analytical Chemistry. A systematic study of the effect of deuteration on retention time and an optimization of stationary and liquid phases to reduce the effect for better quantitative analysis. https://doi.org/10.1021/acs.analchem.5c00289
#Chromatography #LCMS
#Chromatography #LCMS
Mechanistic Study of the Deuterium Effect in Chromatographic Separation for Chemical-Tagging Metabolomics and Its Application to Biomarker Discovery in Metabolic Dysfunction-Associated Steatohepatitis
Over the past decade, numerous metabolomics techniques have been developed using liquid chromatography–mass spectrometry (LC-MS). These methodologies have yielded significant findings and facilitated the identification of biomarkers. Among these, chemical-tagging methodologies combined with isotope surrogate tags have garnered considerable attention as a leading approach. Chemical-tagging reduces labor and costs by eliminating the need for internal standard preparation. However, the chromatographic deuterium effect (CDE) has persisted as a significant challenge. CDE poses a risk of data misinterpretation in metabolomics due to potential differences in matrix effects. Although the CDE mechanism has been partially elucidated, it has primarily been attributed to differences in hydrophobicity. A detailed understanding of CDE mechanisms would be valuable for designing chemical tags and optimizing liquid chromatography (LC) conditions. Moreover, emphasizing the CDE could aid in the separation and purification of deuterated compounds. In this study, we investigated the mechanistic basis of the CDE. Initially, four chromatography columns with different separation modes─octadecyl, octadecyl with a positively charged surface, biphenyl, and pentafluorophenyl (PFP) groups─were evaluated based on retention differences between 1H- and 2H6-labeled chemically tagged metabolites. Among these, the PFP column demonstrated the most effective reduction of the CDE, suggesting that electronic interactions with fluorine stabilized 2H-labeled metabolites. Further optimization using the PFP column showed its efficacy in reducing the level of CDE in human serum samples. Finally, the optimized approach was successfully applied to global metabolomics analysis of serum from a mouse model of metabolic dysfunction-associated steatohepatitis.
TogaA wee #SwatchWednesday this week.
Now that my Inkvent has arrived (no spoilers!), I've decided to dig out my #Chromatography strips to get a more accurate idea of how they're made up compared to my usual paper towel pseudo-chromatography. You can see how limited the latter is compared to the chromatograms.
The inks here are Tono & Lims Rainy Winter & Space Sunset. They're both chromashading inks with some similar notes but very different end results.
