StructuralBiologySep 16, 2024
A MicroED structure of the MAL TIR-mediated MyD88 TIR higher-order assembly reveals a two-stranded arrangement of TIR domains similar to MAL TIR self-assemblies #MicroED #MyD88 #TIRDomain https://t.co/K0Jt3tVITD
Microcrystal electron diffraction structure of Toll-like receptor 2 TIR-domain-nucleated MyD88 TIR-domain higher-order assembly

This study addresses the structural basis of TIR-domain-mediated signal transduction in Toll-like receptor 2 pathways. It is demonstrated that TLR2, but not TLR1 or TLR6, TIR domains induce the formation of MyD88 TIR-domain assemblies in vitro. The MicroED structure of TLR2-induced MyD88 TIR assemblies determined at 2.85 Å resolution highlights conformational changes that are crucial for signaling and provides evidence that TLR2 can directly interact with MyD88 to initiate TLR signaling.

Acta Crystallographica Section D
SBGridNov 2, 2023

Fisk University student Vida Robertson highlighted a publication from SBGrid member Tamir Gonen of University of California, Los Angeles that appeared in Advanced Science, in which the authors describe their use of MicroED to determine the conformational states of the commonly prescribed medication Mirabegron.

https://www.tumblr.com/sbgridconsortium/732639903900368896/using-microed-for-molecule-conformations?source=share

<ALT= Dog beta3 adrenergic receptor (grey) and Mirabegron (cyan)>

#sbgrid
#structuralbiology
#research
#MicroEd
#science

Using MicroED for molecule conformations

Disclaimer: This publication highlight is part of the SBGrid/Meharry Medical College Communities Project, focused on science education and demonstrating how structural biology and preclinical science…

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QuantifoilApr 3, 2023
Fantastic to see #cryoEM #MicroED having an impact on problems that have otherwise resisted investigation by #crystallography in this new pre-print from Cody Gillman in Tamir Gonen's lab at UCLA. With the help of molecular docking simulations suggest a mechanism of action where a non-proteinaceous toxin adopts a peptide-reminiscent hairpin structure to block the channel.
https://www.biorxiv.org/content/10.1101/2023.03.31.53516
DrSarahEJBowmanMar 18, 2023

Excited to be presenting a Thermo Fisher Scientific Ask the Experts webinar 3/22/23 11am EDT with Devrim Acehan!

We'll discuss emerging methods in cryo-EM of microcrystals, as well as our collaborative work as we seek to further develop microcrystal detection and handling methods.

Register here: https://tinyurl.com/2p9d9zer

www.getacrystal.org
www.cryo-EM.center

#structuralbiology #crystallization #cryoem #microED #webinar
@strucbio

Cryo EM | Life Science Research | Thermo Fisher Scientific - DE

electron microscopy (cryo EM) researchers discuss the practical and theoretical aspects of electron microscopy applications and techniques.

getacrystal 💎Nov 9, 2022

Hi from the National High-Throughput Crystallization (HTX) Center in #Buffalo NY.

The HTX Center specializes in #crystallization 💎 for #StructuralBiology.

We are a team of 👩🏻‍🔬👩🏼‍🔬 👩🏽‍🔬 👩🏾‍🔬 and will often post #crystal images, #robot images, and updates for HTX Center users.

www.getacrystal.org

#introduction #toot

#Crystallography #XrayCrystallography #NeutronCrystallography #XFEL #microED

DrSarahEJBowmanNov 2, 2022

Hi all - new to #Mastodon (fleeing the 🦃 #TwitterMigration).

I'm an Associate Investigator & #StructuralBiologist at HWI in Buffalo #NY. I direct the National High-Throughput #Crystallization Center 💎.

My group uses X-ray #crystallography, #microED, #computationalbiology, & other #biophysical tools to study #protein structure & function.

Especially interested in #metalloproteins & coupling #crystallography & #spectroscopy.

#Introduction #Science #SciComm #structuralbiology #WomeninSTEM