@gpollara @JExpMed Intriguing. In an earlier postdoc position I had a look at #DendriticCells, and especially the #cDC1 were abundant, but seemed to be just sitting in the inflammatory environment, doing little. One of the very abundant cytokines: #IL6
We also looked at the effect of that and other #cytokines, but mostly on #Tcells, among others due to the low abundance of cDC1 in HC blood to be able to do many in vitro experiments.
"Mechanistically, glycolysis augmented ATP production to boost STING activation and STING-dependent DC antitumor functions. Moreover, DC-intrinsic STING activation accelerated HIF-1a–mediated glycolysis and established a positive feedback loop."
In the middle of an excellent seminar in which Matthias Gromeier presented work on Cancer Immunotherapy with Recombinant Poliovirus, the Zoom session was ended and no luck from the organization's side to restart. 😭
The first 10-15min showed work linking back to original work and ideas from Albert Sabin and David Bodian. Specific targeting of #DendriticCells and #Macrophages, #interferon response.
Hopefully they recorded the seminar.
Last week, the paper based on my work in the Wilhelmina Children's Hospital (WKZ) was published, with the title "Conventional dendritic cells type 1 are strongly enriched, quiescent and relatively tolerogenic in local inflammatory arthritis".
https://doi.org/10.3389/fimmu.2022.1101999
Credits to Anoushka Samat for taking care of this after I left the department. Without her this work would not be out there. And of course thanks to the rest of the team.
#immunology #RNAseq #inflammation #DendriticCells #monocytes
IntroductionDendritic cells (DC) are crucial for initiating and shaping immune responses. So far, little is known about the functional specialization of human DC subsets in (local) inflammatory conditions. We profiled conventional (c)DC1, cDC2 and monocytes based on phenotype, transcriptome and function from a local inflammatory site, namely synovial fluid (SF) from patients suffering from a chronic inflammatory condition, Juvenile Idiopathic Arthritis (JIA) as well as patients with rheumatoid arthritis (RA).MethodsPaired PB and SF samples from 32 JIA and 4 RA patients were collected for mononuclear cell isolation. Flow cytometry was done for definition of antigen presenting cell (APC) subsets. Cell sorting was done on the FACSAria II or III. RNA sequencing was done on SF APC subsets. Proliferation assays were done on co-cultures after CD3 magnetic activated cell sorting (MACS). APC Toll-like receptor (TLR) stimulation was done using Pam3CSK4, Poly(I:C), LPS, CpG-A and R848. Cytokine production was measured by Luminex.ResultscDC1, a relatively small DC subset in blood, are strongly enriched in SF, and showed a quiescent immune signature without a clear inflammatory profile, low expression of pathogen recognition receptors (PRRs), chemokine and cytokine receptors, and poor induction of T cell proliferation and cytokine production, but selective production of IFNλ upon polyinosinic:polycytidylic acid exposure. In stark contrast, cDC2 and monocytes from the same environment, ...
Check our last contribution to study #CellMigration in #DendriticCells, by #LolaH and @LpzLpz_Tami
(Thanks to @renkawitzteam for the invitation)
There is a lot more assays to have fun !
Full @EurJImmunol: https://onlinelibrary.wiley.com/doi/full/10.1002/eji.202249925
Y’all, this thread by @jeffgilchrist is so important and good. All about #Covid and our emerging understanding of its deleterious effects on #immunity.
Attached: 1 image What is going on with our immune cells after COVID-19 infection? This thread will explore some of the impacts of #COVID #infection on our #immune system even after people are recovered including: - Missing naive #T-cells - Exhausted T-cells - Loss of #B-cell maturation - Hyper-activated #inflammatory response - Immune system is impeded post-infection - #Immunological #dysfunction persists 8+ months - #Depletion and dysfunction of #dendritic cells 🧵 1/
Dear people interested in #organoids #Tcells #Macrophages #Bcells #DendriticCells #MucosalImmunology #microbiome #OrganOnChip #MicroPhysiologicalSystems #IBD #CeliacDisease #iPSCs,
where are you? I want to follow you!
Best regards,
Me
halama_immuno
Arjan Boltjes
Thiago Carvalho
CellComM Lab
CrisBonorino
Dr. Orna Izakson ND, RH(AHG)
Joram Mooiweer