Jocelyn EtienneInteresting : In a #fibrin #gel, inseritng 5% inclusions lead to a 10× increase of #shearModulus and switch from softening to stiffening behaviour. #Collagen gels also show some of these features.
micronicle.orgExperimenting with #ThT stain on #spider webs to see if it successfully bonds to the beta chain #fibrin / #amyloid like components. I've applied the stain and naturally under 460nm webs fluoresce the expected green. I'm waiting for the sample to completely dry before differentiating with alcohol and then using a water based mount to finally disassociate the unbounded stain from the webs and other artefacts. All being so, we might be left with ThT stained #spidroins #science #biology #microscopy
Christophe VeltsosInteresting 🧵 by Nick Anderegg going through a study about clots, Fibrin, and SARS-CoV-2:
threadreaderapp.com/thread/18290...
#Clots #Covid #Fibrin
Thread by @NickAnderegg on Thr...
Thread by @NickAnderegg on Thr...
MEActNOWJust published #Nature
How #Fibrin drives brain inflammation in acute and #LongCovid and that a monoclonal antibody to fibrin protects against microglial activation
https://www.nature.com/articles/s41586-024-07873-4
#MedSpout
trendless> Although the symptoms of Long COVID are multifarious, we here argue that the ability of these fibrin amyloid microclots (fibrinaloids) to block up capillaries, and thus to limit the passage of red blood cells and hence O2 exchange, can actually underpin the majority of these symptoms. Consistent with this, in a preliminary report, it has been shown that suitable and closely monitored ‘triple’ anticoagulant therapy that leads to the removal of the microclots also removes the other symptoms.
A central role for amyloid fibrin microclots in long COVID/PASC: origins and therapeutic implications
Post-acute sequelae of COVID (PASC), usually referred to as ‘Long COVID’ (a phenotype of COVID-19), is a relatively frequent consequence of SARS-CoV-2 infection, in which symptoms such as breathlessness, fatigue, ‘brain fog’, tissue damage, inflammation, and coagulopathies (dysfunctions of the blood coagulation system) persist long after the initial infection. It bears similarities to other post-viral syndromes, and to myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS). Many regulatory health bodies still do not recognize this syndrome as a separate disease entity, and refer to it under the broad terminology of ‘COVID’, although its demographics are quite different from those of acute COVID-19. A few years ago, we discovered that fibrinogen in blood can clot into an anomalous ‘amyloid’ form of fibrin that (like other β-rich amyloids and prions) is relatively resistant to proteolysis (fibrinolysis). The result, as is strongly manifested in platelet-poor plasma (PPP) of individuals with Long COVID, is extensive fibrin amyloid microclots that can persist, can entrap other proteins, and that may lead to the production of various autoantibodies. These microclots are more-or-less easily measured in PPP with the stain thioflavin T and a simple fluorescence microscope. Although the symptoms of Long COVID are multifarious, we here argue that the ability of these fibrin amyloid microclots (fibrinaloids) to block up capillaries, and thus to limit the passage of red blood cells and hence O2 exchange, can actually underpin the majority of these symptoms. Consistent with this, in a preliminary report, it has been shown that suitable and closely monitored ‘triple’ anticoagulant therapy that leads to the removal of the microclots also removes the other symptoms. Fibrin amyloid microclots represent a novel and potentially important target for both the understanding and treatment of Long COVID and related disorders.
Giuseppe Michieli#SARS-CoV-2 #infection of #human #lung epithelial cells induces TMPRSS-mediated acute #fibrin deposition https://www.nature.com/articles/s41467-023-42140-6 #research #library #pathology
SARS-CoV-2 infection of human lung epithelial cells induces TMPRSS-mediated acute fibrin deposition - Nature Communications
Severe SARS-CoV-2 infection has been associated with extensive diffuse alveolar damage and fibrin formation. Here, Erickson et al describe an infection-induced coagulation mechanism which involves activation of prothrombin by members of TMPRSS genes.
@DysautonomiaZA - I know a bunch of #mecfs and #longcovid patients who got their testing done in Germany. #TeamClots found that all #pwLC and pwME had these #Fibrin and #Fibrinogen #microclots.
Many of us have been taking #NattoSerra blend enzymes that help reduce microclots.
It has really helped me. MCAS patients, it has hitamine so lumbrokinase might be a better option.
NIH has published studies about these enzymes if anyone is interested.
Rhyothemis@shanaqui
There's another talk - I barely remember it - but IIRC it was interesting. Apparently mast cells can also release extracellular traps.
Presented by Imre Varju, MD, PhD
"Immune-derived extracellular traps in COVID-19 and other infections" Sept 2020
https://youtu.be/avFT3ypEBas
#immunology #COVID19 #neutrophils #MastCells #mitochondria #NETosis #ExtracellularTraps #thrombosis #clotting #fibrin
#myeloperoxidase
#histones
Immune-derived extracellular traps in COVID-19 and other infections
hackaday unofficialIt’s Printable, It’s Programmable, It’s E. coli
Well, whaddya know? It seems that E. coli, the bane of Romaine and spinach everywhere, has at least one practical use. Researchers at Harvard have created a kind of 3D-printable ink that is alive and made entirely of microbes produced by E. coli. Although this is not the first so-called living ink, it does hold the title of the first living ink that doesn't need any additional polymers to provide structure.
Passing the pillar test up to 16mm. Image via Nature
Because the ink is alive, it is technically programmable in the sense that it can self-assemble proteins into nanofibers, and further assemble those into nanofiber networks that comprise hydrogels.
One of the researchers compared the ink to a seed, which has everything it needs to eventually grow into a glorious tree. In this way, the ink could be used as a renewable building material both on Earth and in space. Though the ink does not continue to grow after being printed, the resulting structure would be a living system that could theoretically heal itself.
The ink creation process begins when the researchers induce genetically-engineered bacteria cultures to grow the ink, which is also made of living cells. The ink is then harvested and becomes gelatin-like, holding its shape well enough to go through a 3D printer. It even passes the bridging test, supporting its own weight between pillars placed up to 16 mm apart. (We'd like to see a Benchie.)
Microbial Mechanics
Is it weird to work with microbes? Not really. They are already a part of vitamins and some perfumes, and other types of microbes are out there maturing into biodegradable plastics. E. coli has been used before to create a hydrogel capable of growing and regeneration, but it wasn't viscous enough to pass through a printer. So they genetically engineered the E. coli to produce a natural polymer from fibrin, which is a blood-clotting polymer possessed by humans and animals alike. The polymer created by the bacteria is designed to link together into a network, which one researcher likened to a heavy-duty cargo net.
This all-microbe E. ink is still being developed. So far, it is already quite an improvement over what was viable only five years ago. Why 3D prints with E. coli? The ink showed promise as a drug delivery system, for example. In a test, the ink was shown to release an anti-cancer drug called azurin after being exposed to a certain chemical. One of those functions might be cleaning up the environment. In another test, the ink was proven to sequester the toxin BPA from its surroundings.
Though this all sounds great, the ink is still in development and has a way to go. For one thing, it doesn't withstand drying out, but that may not be an obstacle if the ink is combined with tissue engineering. However, if we're going to use it as a self-regenerating material for building habitats on Earth and in space, it will have to become more stable.
Via [New York Times]
#news #science #biopolymer #ecoli #fibrin #ink #microbes #microbialink #printablebacteria
