Time-resolved STED nanoscopy in life sciences

Time-resolved STED nanoscopy in life sciences

Zooming across time and space simultaneously with superresolution to understand how cells divide

Superresolution microscopy allowed researchers to view cells at the molecular level. Improvements on the technique can help study the building blocks of complex cell processes over time.

Looking deeper with adaptive six-dimensional nanoscopy

With a $2 million NIH grant, Matthew Lew will develop smart microscopes to reveal dynamic interactions between individual biomolecules

Nanoscale rules governing the organization of glutamate receptors in spine synapses are subunit specific - Nature Communications

Glutamate receptors comprise two obligate subunits and two subunits that confer distinct properties and functions to the specific tetramers, which also localize to distinct synaptic spines. Here, the authors use STimulated Emission Depletion nanoscopy (STED) to provide detailed insights into the spatial organization of glutamate receptor types.

Nanoscale rules governing the organization of glutamate receptors in spine synapses are subunit specific - Nature Communications

Glutamate receptors comprise two obligate subunits and two subunits that confer distinct properties and functions to the specific tetramers, which also localize to distinct synaptic spines. Here, the authors use STimulated Emission Depletion nanoscopy (STED) to provide detailed insights into the spatial organization of glutamate receptor types.

Single-Molecule Localization Microscopy: Theoretical Basis and Practical Guide

A Single-Molecule Localization Microscopy (SMLM) lecture for life scientists was given virtually during the “Widening the Lens” Microscopy Education program at the Vanderbilt University, Nashville, TN, USA, on December 7, 2022. During the presentation, I gave the students an overview of practical aspects (sample handling, hardware) and the theoretical basis (image processing) of SMLM with the aim of making them more literate when dealing with such datasets and/or attempting to do their own measurements and analysis.   References of all used figures and videos are given. Please feel free to contact Ulrike Boehm in case of questions.