Leica Microsystems

@leicamicrosystems
185 Followers
29 Following
41 Posts

➑ Discover how innate immunity can sustain deleterious effects following neurovascular pathologies. Follow our new, enlightening webinar on demand and see how technological developments enable longitudinal studies into these events.

➑ Speakers are Thomas Mathivet, Researcher, BRIC - INSERM U1312 - Bordeaux University and Ulf Schwarz, Application Manager (LS), Leica Microsystems

▢️ https://fcld.ly/f2ye86w

#neurovascular #pathologies #immunity #research #science #microscopy

Windows on Neurovascular Pathologies

Discover how innate immunity can sustain deleterious effects following neurovascular pathologies and the technological developments enabling longitudinal studies into these events.

Are these little aliensπŸ‘½ or baby moss plantsπŸͺ΄? πŸ˜„
.
.
.
This collage is a great example of the power of plant regeneration. Each of these individual 7-day-old moss (Physcomitrium patens) plants, was regenerated from a single protoplast (plant cell with removed cell walls).
This is what you see when you zoom in on fungus, growing uninvitedly on my "sterile" moss plates. What a beautiful hyphal network. #Fungi #Beatifulcontamination #Imaging
The image was made with the THUNDER Imager Model Organism dissecting scope (M205FCA) from @leicamicrosystems, available at the WUSTL Biology department imaging core. The image is a stitched composite from 9 (3 by 3) tile images captured using the 1x/0.6 objective with a 6.3x mag and greyscale camera.

βœ” Striving to achieve consistent confluency measurements for your cell culture experiments?
βœ” Help improve experiment reproducibility by using a common criterion to measure confluency for all experiments and researchers. Eliminate subjective estimations with Mateo TL.

βœ” Know more here
πŸ‘‰ https://fcld.ly/f5cl1rd

#MateoTL #digitaltransmittedlight #invertedmicroscope #cellculture

How to do a Proper Cell Culture Quick Check

In order to successfully work with mammalian cell lines, they must be grown under controlled conditions and require their own specific growth medium. In addition, to guarantee consistency their growth must be monitored at regular intervals. This article describes a typical workflow for subculturing an adherent cell line with detailed illustrations of all of the necessary steps.

Going to GSA (Genetics Society of America) #Dros23? Stop by the Leica Booth 601 to check out our stereomicroscopes! Our friendly Chicago team will be there to chat with you about Leica imaging solutions.
.
.
βœ… More information πŸ‘‰ https://genetics-gsa.org/drosophila-2023/
.
.
πŸ“Ή Video description: Drosophila follicle cells stained with markers to examine apical basal polarity, before and after THUNDER processing. Images courtesy of Mark Khoury and David Bilder.
.
.
.
#drosophila #cells #gsa
Homepage

64th Annual Drosophila Research Conference

βœ… Increase your slide scanning experiment speed and perform fast and efficient multicolor acquisition. You'll be able to assess more markers with less photobleaching. πŸ™Œ
πŸ‘‰ https://fcld.ly/5clrpvv

#THUNDER #THUNDERImager #widefield #lifesciences

THUNDER Imager Tissue

The THUNDER Imager Tissue allows real-time fluorescence imaging of 3D tissue sections typically used in neuroscience and histology research.

β€œWe are proud to announce that Leica Microsystems has partnered with Elles bougent in France." πŸ™Œ
🌈 As part of Diversity, Equity, and Inclusion efforts, we aim to support female students in their education choice and careers by breaking codes and stereotypes. We are thankful πŸ™ for the opportunity to actively involve our associates and teams in this partnership.
πŸ‘‰ https://fcld.ly/w5kq8vq

#EllesBougent #Diversity #Inclusion #wonderwhatwithin #youngtalent #female #students #diversetalents

Nos partenaires - Leica Microsystems

Elles bougent

πŸ”Ά What are the additional insights you can gain from imaging tissue sample in 10 colors?
πŸ”Ά Follow our on-demand webinar with Dr. Nicolas Gaudenzio and discover how to perform 10-color imaging using a confocal microscope. See how this allows to assess the skin immune landscape.

https://fcld.ly/lciceh8

#STED #STELLARIS #confocal #immune

Confocal Imaging of Immune Cells in Tissue Samples

In this webinar, you will discover how to perform 10-color acquisition using a confocal microscope. The challenges of imaged-based approaches to identify skin immune cells. A new pipeline to assess skin immune landscape in both healthy and pathological samples.

πŸ“„ Read this article to see how diseases related to scaffold proteins, like Daple which regulates crosstalk in G-protein signaling, can be studied efficiently in zebrafish models with a THUNDER Imager.
πŸ‘‰ https://fcld.ly/sisokgn

πŸ–ΌοΈ EDoF projections of a GFP-tagged zebrafish fin: A) raw widefield and B) LVCC processed images. Images are courtesy of the Jason Ear lab at Cal Poly Pomona, California, USA.

#THUNDER #Imager #science #reserach #proteins #widefield #fluorescence #microscopy

Diseases Linked to Scaffold Proteins and Signaling

This article shows how diseases related to scaffold proteins and protein signaling can be studied in zebrafish models efficiently with a THUNDER Imager.

Fluorescein isothiocyanate (FITC) is an organic fluorescent dye and probably one of the most commonly used in #immunofluorescence and flow cytometry.
Learn more about Fluorescent Dyes: https://fcld.ly/b5zd1oh

πŸ–ΌοΈ Drosophila embryo development, Green: FITC, Red: TRITC.

#fluorescencefriday #fluorescence #dyes #microscopy #science #sciencemastodon

Fluorescent Dyes

A basic principle in fluorescence microscopy is the highly specific visualization of cellular components with the help of a fluorescent agent. This can be a fluorescent protein – for example GFP – genetically linked to the protein of interest. If cloning is impossible – for instance in histologic samples – techniques such as immunofluorescence staining are used to visualize the protein of interest.