Time-resolved STED nanoscopy in life sciences

Ultrastructure expansion microscopy reveals the cellular architecture of budding and fission yeast

Summary: The resolution limit of visible light has limited ultrastructure imaging in microbes. We adapt U-ExM for budding and fission yeast, thereby improving the accessibility of super-resolution microscopy of these powerful genetic models.

First person – Kerstin Hinterndorfer and Felix Mikus

ABSTRACT. First Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping researchers promote themselves alongside their papers. Kerstin Hinterndorfer and Felix Mikus are co-first authors on ‘ Ultrastructure expansion microscopy reveals the cellular architecture of budding and fission yeast’, published in JCS. Kerstin is a PhD student in the lab of Robbie Loewith at University of Geneva, Switzerland, working on TOR signaling and expansion microscopy in yeast. Felix is a predoctoral fellow in the lab of Dr Gautam Dey in Heidelberg, Germany, investigating how nuclear pore complex dynamics regulate nuclear remodeling and proteostasis in closed and semi-closed mitosis.

Lipid expansion microscopy uses 'power of click chemistry'

Lipids—fats—make great walls for cells and organelles because they are water resistant and dynamic. But those same characteristics also make them hard to image using expansion microscopy, a technique that works for magnifying other cell components.