hi #fediverse. I got a #mRNA transfection question. for a long mRNA (say a few thousand nucleotides), fully condensed, it quickly become >20 nm in size. Putting that in a #lipid #nanoparticle will make it grow even larger, and you get different cellular uptake mechanisms.
Who can point me to some good literature? (yeah, I will do some searching myself, but you are all so awesome and don't want to miss out on your expertise ;)
New paper just out:
*Effects of Constant and Time-Varying Colloidal Stability on Metal Oxide Nanoparticle Agglomeration: Insights from Kinetic Monte Carlo Simulations*
https://doi.org/10.1016/j.ces.2026.123814
The research presented in this paper was undertaken with funding from UK Research and Innovation (UKRI), grant number EP/X022218/1, as part of the UK Japan Civil Nuclear Energy Collaboration Phase 9.
Researchers at MIT have just developed a new lipid nanoparticle that super-enhances the effectiveness of the mRNA vaccine in mice to a hundred times its stand-alone effectiveness, thus offering massive savings by reducing required dosages while also reducing toxicity in the liver.
Recent technological advances are fueling the development of cutting-edge technologies that can monitor and control physiological processes with high precision. These include devices that could control the expression of genes within living organisms, without requiring invasive surgeries or procedures.
15th Workshop on X-Ray Nano-Imaging of Biological and Chemical Systems at PETRA III
βThe workshop gives attention to x-ray #microscopy at various PETRA III beamlines with special emphasis on
applications in #biology and #chemistry. Several scientific (multimodal) #XRay #imaging highlights are presented
with focus on the scanning X-ray microscopy beamlines P06, P10 and P05.β
β Diamond-CRLs
β Multibeam #Ptychography
β #Nanoparticle distribution
β #Nanoscopy: #semiconductors
#Immunogenicity & #biodistribution of lipid #nanoparticle formulated self-amplifying #mRNA #vaccines against #H5 avian #influenza, npj Vaccines: https://www.nature.com/articles/s41541-024-00932-x
Cellular immune responses & #antigen-specific #IgA antibodies in #lungs were also observed. Comparison of sa-mRNA vaccines encoding secreted & membrane-anchored full-length HA revealed that anchoring of HA to the membrane significantly enhanced the antibody and cellular responses.
This study reports on the immunogenicity and biodistribution of H5 hemagglutinin (HA)-based self-amplifying (sa) mRNA vaccines in mice. Four sa-mRNA vaccines encoding either a secreted full-length HA, a secreted HA head domain, a secreted HA stalk domain, or a full-length membrane-anchored HA were investigated. All vaccines elicited an adaptive immune response. However, the full-length HA sa-RNA vaccines demonstrated superior performance compared to head and stalk domain vaccines. The antibody titers positively correlated with the vaccine dose. Cellular immune responses and antigen-specific IgA antibodies in the lungs were also observed. The comparison of the sa-mRNA vaccines encoding the secreted and membrane-anchored full-length HA revealed that anchoring of the HA to the membrane significantly enhanced the antibody and cellular responses. In addition to the injection site, the intramuscularly injected sa-mRNA-LNPs were also detected in the draining lymph nodes, spleen, and to a lesser extent, in the lung, kidney, liver, and heart.
Lithium Battery for Electric Vehicles (EVs): Separators with coatings of Silica Nanoparticles enhance Safety
Lithium-ion batteries for electric vehicles (EVs) face safety and stabilityissues due to overheating of separators, short circuits and reduced...........
#ElectricVehicles #EV #Lithium #LithiumBattery #Nanoparticle #Separators
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