🧠 New pre-print by Wiesner et al. (2025) shows non-#synaptic #exocytosis directly from the #axon shaft, regulated by the submembrane periodic skeleton. Using #superresolution #imaging and live assays (#HiLo (VAMP2-pHluorin), #SIM, and correlative two-color #SMLM/ #STORM) they reveal that #axons can release vesicles outside classical #synapses, expanding how we understand #neuronal communication and #AxonalSignaling.
Congratulations to Prof. Jin Lu, now at the National Center for Nanoscience, whose work on #singlemolecule #electrochemical imaging is now online in Chemical Science. Happy New Year!
#SMLM #WashU #WUSTL @chemicalscience #ChemicalScience
"Single-Molecule Electrochemical Imaging Resolves the Midpoint Potentials of Individual Fluorophores on Nanoporous Antimony-Doped Tin Oxide"
Chemical Science, 2024
https://doi.org/10.1039/D3SC05293A
Very proud of this work of Clément Cabriel, finally published in Nature Photonics!
Event-based vision sensors are here to stay and conquer your hearts and #SMLM microscopes.
Article here: https://www.nature.com/articles/s41566-023-01308-8
Full text access here: https://rdcu.be/dpQhX
Event-based sensors enable super-resolution single-molecule localization microscopy with comparable quality and resolution to traditional scientific cameras, while also overcoming the limitations of high-density imaging.
Are there any microscopists hiding here?
Adding :AddBiophysicist to this post in order to get in the "Biophysicists on Mastodon" list...
Cost-efficient #OpenSource #laser engine for #microscopy:
-405 nm, 488 nm & 638 nm and optionally a 561 nm diode-pumped solid-state laser
-agitated multimode fiber to suppress speckles
https://doi.org/10.1364/boe.380815
#DIYbio #hackerspace #lab #instruments #SMLM #microscope #imaging
An #OpenSource, high resolution, #automated #fluorescence #microscope:
-reaches single molecule sensitivity
-single particle tracking and #SMLM super-resolution microscopy experiments in cells
https://doi.org/10.1101/2023.05.31.542706
#DIYbio #lab #instruments #optics #imaging
Team achieves Ångström-resolution #fluorescence #microscopy.
https://phys.org/news/2023-05-team-ngstrm-resolution-fluorescence-microscopy.html
A breakthrough in fluorescence microscopy has been achieved by the research group of Ralf Jungmann at the Max Planck Institute of Biochemistry (MPIB) and Ludwig-Maximilians-Universität (LMU) Munich. The team developed Resolution Enhancement by Sequential Imaging (RESI), a revolutionary technique that enhances the resolution of fluorescence microscopy down to the Ångström scale. This innovation is poised to usher in a paradigm shift in our approach to study biological systems with thus far unprecedented detail.
Fabrizio Musacchio
Lew Lab @ WashU ✅
Bose-Einstein-Kondensat
Ignacio Izeddin
CellBioNews
AI4Life