Ars Technica NewsDNA and jolts of electricity get people to make optimal antibodies https://arstechni.ca/eBs6 #electroporation #antibodies #medicine #Science #Biology #plasmid #Policy #DNA
ESCMIDExcited to kick off #IMMEMXIV in Porto, Portugal with the first session focusing on #plasmid typing for surveillance! A fantastic session thanks to our 5 great speakers and now we are going into the first debate session on strain typing in high-income vs low-resource settings. #IDSky #clinmicro
Konsole Kombat Podcast#gamers! This week's episode is a fight a long time coming, as we find out who would win between #Dishonored protagonist #corvoattano and #Bioshock protagonist #jackwynand! Outsider #Magic meets #Plasmid Powers! Wanna know who wins? Tune in now! #podcast #gaming #fyp #explorepage
PLOS BiologyHow best to avoid the costs of bearing a plasmid? @rctwright @jpjhall &co find it's best for #bacteria to compensate plasmids by chromosomal (not plasmid-borne) mutations, resulting in 'hubs' for #plasmid accumulation evolution & dissemination #PLOSBiology https://plos.io/3ODbXdN
A chromosomal mutation is superior to a plasmid-encoded mutation for plasmid fitness cost compensation
Plasmids spread important traits in microbiomes but impose fitness costs on their hosts. This study of the ecological consequences of plasmid-bacterial compensatory evolution reveals benefits to bacteria that compensate plasmids by chromosomal (rather than plasmid-borne) mutations, resulting in ’hubs’ for plasmid accumulation, evolution, and dissemination.
charrveinAMR Evolution, Conjugative Plasmids, Phage Therapy, and CRISPR Antimicrobials
Ilmur Jónsdóttir gave a lecture on her new phd thesis
#bacteria #amr #resistance #crispr #plasmid #phages #evolution
Cosmin SaveanuBecoming a big fan of whole #plasmid sequencing for new constructs (Nanopore). Also a big fan of Q5 and Phusion polymerases for their accuracy and of the Hot Fusion variant of the Gibson assembly for its price and convenience (no ligation required!)
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0115318
Hot Fusion: An Efficient Method to Clone Multiple DNA Fragments as Well as Inverted Repeats without Ligase
Molecular cloning is utilized in nearly every facet of biological and medical research. We have developed a method, termed Hot Fusion, to efficiently clone one or multiple DNA fragments into plasmid vectors without the use of ligase. The method is directional, produces seamless junctions and is not dependent on the availability of restriction sites for inserts. Fragments are assembled based on shared homology regions of 17–30 bp at the junctions, which greatly simplifies the construct design. Hot Fusion is carried out in a one-step, single tube reaction at 50°C for one hour followed by cooling to room temperature. In addition to its utility for multi-fragment assembly Hot Fusion provides a highly efficient method for cloning DNA fragments containing inverted repeats for applications such as RNAi. The overall cloning efficiency is in the order of 90–95%.
micrordtUnveiling the Versatile World of Plasmids: Nature’s Tiny Genetic Gems
#ApplicationsOfPlasmid, #Bacteria, #BacterialPlasmid, #DNA, #Plasmid, #PlasmidFingerprinting, #PlasmidIsolation #Genetics, #MolecularBiology, #RDT In the realm of genetics, plasmids stand as miniature marvels, often overshadowed by the grandeur of chromosomes yet wielding immense…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -
Isolation of Plasmids from Bacterial Cells: A Step-by-Step Guide
#Bacteria, #CellLysis, #Plasmid, #PlasmidIsolation #Bacteriology Introduction Plasmids are small, circular pieces of DNA that are separate from the chromosomal DNA in bacteria. They play a…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -
Reproduction in Bacteria: A Fascinating World of Rapid Multiplication
#Bacteria, #BinaryFission, #Mutation, #Plasmid, #Reproduction, #ReproductionInBacteria #Bacteriology Introduction Bacteria are among the oldest and most abundant life forms on Earth. Despite their small size and simplicity, they…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -
Extra Chromosomal Genetic Element (Plasmid)
#Conjugation, #GeneTransfer, #GeneralizedTransduction, #Plasmid, #SpecializedTransduction, #Transduction, #Transformation #Genetics In addition to the normal DNA some bacteria also have extra chromosomal DNA (i.e. DNA other then present in chromosomes)…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -
https://micrordt.wordpress.com/2024/05/04/extra-chromosomal-genetic-element-plasmid/
