PsychoticSheepThrowback to one of my favorite lab successes from last year! 🧬
Image 1: The most beautiful pellet. Purified plasmid DNA, still in the wash solution, isolated via midiprep from E. coli that I cloned myself.
Image 2: Pure lab chaos. In the middle of the midiprep.
Image 3: The proof. 3158 ng/µL of isolated clean DNA measured on the Nanodrop. Yes, that is much xD
Image 4: And the happy ending. The gel image shows the successful transformation of the gene into Acetobacterium woodii. The new gene was inserted using CRISPR Cas via homologous recombination. Project goal achieved! Two new A. woodii strains that didn't exist before I created them. Proud parent moment. 🧬✂️
The goal of the project is to use A. woodii to produce bioalcohols like ethanol or 2‑butanol (that's my area) directly from CO₂ in the air. 🌍💚
#LabLife #MolecularBiology #CRISPR #GenomeEditing #Microbiology #Science #Research #Plasmid #Biotechnology
Image 1: The most beautiful pellet. Purified plasmid DNA, still in the wash solution, isolated via midiprep from E. coli that I cloned myself.
Image 2: Pure lab chaos. In the middle of the midiprep.
Image 3: The proof. 3158 ng/µL of isolated clean DNA measured on the Nanodrop. Yes, that is much xD
Image 4: And the happy ending. The gel image shows the successful transformation of the gene into Acetobacterium woodii. The new gene was inserted using CRISPR Cas via homologous recombination. Project goal achieved! Two new A. woodii strains that didn't exist before I created them. Proud parent moment. 🧬✂️
The goal of the project is to use A. woodii to produce bioalcohols like ethanol or 2‑butanol (that's my area) directly from CO₂ in the air. 🌍💚
#LabLife #MolecularBiology #CRISPR #GenomeEditing #Microbiology #Science #Research #Plasmid #Biotechnology
