انجاز طبي تونسي الأول من نوعه في العالم.. التفاصيل (فيديو)
> أكد رئيس قسم أمراض #القلب بمستشفى الرابطة محمد سامي مورالي اليوم أن الطاقم #الطبي بالمستشفى تمكن بنجاح من انجاز تدخل #طبي دقيق لمريض يبلغ من العمر 50 عاما وهو الأول من نوعه في #تونس وأيضا في العالم، وفق قوله.
#الكتروبورايشن
#Sante #electroporation #Tunisie
Electric Eels’ Shocking Ability To Alter The Genetics Of Nearby Animals
by @grrlscientist via @medium
#electroporation #eel #fish #evolution #ecology #SciComm https://grrlscientist.medium.com/electric-eels-shocking-ability-to-alter-the-genetics-of-nearby-animals-12c11e9092aa
"I realized that electric eels in the Amazon River could well act as a power source, organisms living in the surrounding area could act as recipient cells, and environmental DNA fragments released into the water would become foreign genes, causing genetic recombination in the surrounding organisms because of electric discharge.” (mismatching quotes sic)
https://www.nagoya-u.ac.jp/researchinfo/result-en/2023/12/20231207-01.html
Refuting the standard model of #electroporation.
https://phys.org/news/2023-03-refuting-standard-electroporation.html
Strong electric fields can be used to create pores in biomembranes. The method is known as electroporation. Inducing such defects in membranes in a targeted manner is an important technique in medicine and biotechnology, but also in the treatment of foodstuffs.
The study of clinically relevant bacterial pathogens relies on molecular and genetic approaches. However, the generally low transformation frequency among natural isolates poses technical hurdles to widely applying common methods in molecular biology, including transformation of large constructs, chromosomal genetic manipulation, and dense mutant library construction. Here we demonstrate that culturing clinical isolates in the presence of polymyxin B nonapeptide (PMBN) improves their transformation frequency via electroporation by up to 100-fold in a dose-dependent and reversible manner. The effect was observed for PMBN-binding uropathogenic Escherichia coli (UPEC) and Salmonella enterica strains but not naturally polymyxin resistant Proteus mirabilis. Using our PMBN electroporation method we show efficient delivery of large plasmid constructs into UPEC, which otherwise failed using a conventional electroporation protocol. Moreover, we show a fivefold increase in the yield of engineered mutant colonies obtained in S. enterica with the widely used lambda-Red recombineering method, when cells are cultured in the presence of PMBN. Lastly, we demonstrate that PMBN treatment can enhance the delivery of DNA-transposase complexes into UPEC and increase transposon mutant yield by eightfold when constructing Transposon Insertion Sequencing (TIS) libraries. Therefore, PMBN can be used as a powerful electropermeabilisation adjuvant to aid the delivery of DNA and DNA–protein complexes into clinically important bacteria.
Ars Technica News
Carthage
GrrlScientist ⧖ Ⓥ SciComm 🇺🇦
Simon Forman
CellBioNews
Universität Freiburg
Vivek Mutalik