A true tour-de-force study by the group of Bernhard Küster posted as bioRxiv preprint. They profiled 133 kinase inhibitors in 5 cell lines at 11 doses using #phosphoproteomics with #decryptM to identify 5,318 confident kinase::substrate relationships for 96 human #kinases. https://www.biorxiv.org/content/10.1101/2025.11.18.689017v1

#Chemistry #ChemBio #Proteomics #DrugDiscovery #Signalling

Our new preprint, presenting PulsPhos, a novel method for proteome-wide measurement of the dynamics of protein phosphorylation at specific sites in living cells, is now out!

This required inventing an elegant strategy for measuring the pool of available phosphates in, which allows us to account for the complex biotransformations of phosphates that occur in intact cells.

https://www.biorxiv.org/content/10.1101/2024.07.23.604744v1

#proteomics #phosphoproteomics #massspec
#bioinformatics

#Phosphoproteomics reveal pervasive rewiring of #Cdk1 and Polo kinase phosphorylations during yeast #meiosis, ensuring the occurrence of two successive divisions without intervening S-phase

Lori Koch, Adele Marston et al

https://www.embopress.org/doi/full/10.1038/s44318-024-00059-8

Global #phosphoproteomics reveal the diverse roles of #casein_kinase 1 in #plant #development.

#CK1

https://phys.org/news/2023-09-global-phosphoproteomics-reveal-diverse-roles.html

Discovering the dynamic world of the EGFR-MAPK phosphoproteome. 🌐🔎

Feng, Sanford and colleagues #EMSLscience developed a multiplexed deep phosphoproteome profiling workflow unveiling 4500 protein sites exhibiting increased phosphorylation upon EGF stimulation.

Another great #preLight by Benjamin Maier 👉 https://prelights.biologists.com/highlights/a-phosphoproteomics-data-resource-for-systems-level-modeling-of-kinase-signaling-networks-v2/

#preprint # modelling #phosphoproteomics #signalling #bioinformatics #SystemsBiology