Mastodawn

Hint: remember that you can use gnomAD to estimate the number of SAVs associated with a gene, for example:
https://gnomad.broadinstitute.org/gene/ENSG00000206172?dataset=gnomad_r4

gnomAD

The Genome Aggregation Database (gnomAD) is a resource developed by an international coalition of investigators, with the goal of aggregating and harmonizing both exome and genome sequencing data from a wide variety of large-scale sequencing projects, and making summary data available for the wider scientific community.

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Ron Beavis ❌7h ago

I was tempted to use the notation "O(proteoform)", but since it wasn't in the notes I thought it would be a bit too obscure for most of the class.

Ron Beavis ❌7h ago

#proteomics_101 Estimate the # of proteoforms (order of magnitude) for human GYPA:p, HBA1:p & PRKDC:p due to splice variants, SAVs, raggedness & S/T/N+glycosyl, S/T+phosphoryl & K+ubiquitinyl/SUMOyl/acetyl. Include p-forms caused by branched glycosyl, ubiquitinyl & SUMOyl moeities. Show your work!

Ron Beavis ❌1d ago

#proteomics_101 Answers to yesterday's question about the practical problems involving SAVs:

a) data that can id patients requires big changes to info security;
b) you need to include RNA/DNA info to interpret proteomics data; &
c) creating a whole new field (SN|AVs) is just too damn hard.

Ron Beavis ❌1d ago

Greg Wilson 1d ago

"AI-Powered Tractor Startup Burns Through a Quarter Billion Dollars, Fires All Employees" https://futurism.com/robots-and-machines/ai-tractor-startup-founders Anyone remember WebVan and Pets.com ?

AI-Powered Tractor Startup Burns Through a Quarter Billion Dollars, Fires All Employees in Epic Implosion

Monarch Tractor, which promised to revolutionize farming with its AI-powered farming equipment, is now in ruins.

Futurism

Ron Beavis ❌2d ago

When ever I see a paper claiming to do proteome-wide this, that & the other, genuinely all I want them to do is to pick a couple of interesting examples & do them so well that they can be held up as a standard of good scientific practice.

Ron Beavis ❌2d ago

Az 3d ago

i love how space travel went from Star Trek: The Next Generation to Deep Space 9 in the space of one (1) lunar mission.

Apollo program: boldly going where nobody has been before, everyone is a decorated pilot putting their lives on the line in the most advanced tech.

Artemis II: shitters broken, "I have two versions of Outlook and neither of them are working".

Ron Beavis ❌2d ago

#proteomics_101 List the 3 most important practical problems associated with analyzing human proteomics data for single amino acid variants, other than trivial computational issues.

Ron Beavis ❌2d ago

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Dave Tabb 2d ago

I shoot mass spectrometry and bioinformatics training videos for my YouTube channel, found here:
https://www.youtube.com/@DavidTabb

David Tabb

Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube.

YouTube

Ron Beavis ❌2d ago

David Chisnall (*Now with 50% more sarcasm!*)2d ago

The Anthropic code leak is showing that contrary to claims made by AI sceptics, it is possible for humans to understand the output of LLM code generators on large and complex codebases. This has shown limitations in conventional office chair design and may require some new health and safety rules instituted in places that allow LLM use, due to the large number of reports of people falling out of their chairs laughing.