PLOS BiologySep 16, 2025
Post-transcriptional #GeneRegulation through #m6A deposition is dysregulated in many diseases. In this #PLOSBiology Unsolved Mystery, Samie Jaffrey &co investigate why the m6A writer complex requires multiple subunits and what roles its individual proteins might have in m6A regulation.
https://plos.io/3VjfjWI
Why does the m6A writer complex require so many proteins?

Post-transcriptional gene regulation through m6A deposition is dysregulated in many diseases and is catalyzed by a multi-protein writer complex. This Unsolved Mystery investigates why the writer complex requires multiple subunits and what roles its individual proteins might have in m6A regulation.

Amelia Cervera 🧬Jun 9, 2025

RNA methylation in retrotransposon control.

#Transposons #N6methyladenosine #m6A

https://www.cell.com/trends/genetics/fulltext/S0168-9525(25)00105-2

LeszekApr 11, 2025
beloved #Mettl3 is involved in DNA repair by placing #m6A in #DNA
#modifications
https://elifesciences.org/articles/101626
N6-methyladenosine in DNA promotes genome stability

Single-cell imaging and mass spectrometry data reveal adenosine in the DNA of mammalian cells can be methylated by METTL3 to form N6-methyladenosine in response to DNA damage.

eLife
The EMBO JournalDec 18, 2023
An #m6A-independent function of #YTHDC1: its expression delays cellular #senescence and pulmonary #fibrosis by interacting with #TopBP1 to activate ATR
https://www.embopress.org/doi/full/10.1038/s44318-023-00003-2
Show threadLeszekNov 15, 2023

More good news: coincidentally (or not) #nanopore release new dorado v0.43 with support for #m6A in DRACH context for RNA004
https://github.com/nanoporetech/dorado/releases/tag/v0.4.3

RNA model is called: [email protected]_m6A_DRACH@v1

Binaries for Linux are available through their ONT community (website still not updated): https://cdn.oxfordnanoportal.com/software/analysis/dorado-0.4.3-linux-x64.tar.gz

Release v0.4.3 Β· nanoporetech/dorado

[0.4.3] (14 Nov 2023) This release of Dorado introduces a new RNA m6A modified base model and initial support for poly(A)/poly(T) tail length estimation. It also introduces duplex performance enhan...

GitHub
LeszekNov 14, 2023

Can you imagine calling #m6A modifications from raw #RNA #nanopore reads just with #basecalling?

Let me introduce you to #m6ABasecaller

https://doi.org/10.1101/2023.11.13.566801

Gal Haimovich πŸ˜·πŸ”¬β˜£πŸ³οΈβ€πŸŒˆπŸ³οΈβ€βš§Oct 31, 2023

🎯🐠 I've been away for a while. But here:
I wrote a blog post on "Imaging mRNA modifications in situ"

Featuring: m6AISH-PLA, ARPLA (on #glycoRNA) & DART-FISH.

#smFISH #m6A #RNA #mRNA #RNAmodifications #microscopy #imaging #singlemolecule

https://greenfluorescentblog.wordpress.com/2023/10/31/imaging-mrna-modifications-in-situ/

Imaging mRNA modifications in situ

There are at least 170 known chemical modifications on RNA molecules in all organisms. Most of these modifications are on tRNAs, rRNAs and small RNAs. But over the past two decades, researchers fou…

greenfluorescentblog
The EMBO JournalSep 18, 2023

In our issue 18:
Reviews on ubiquitin-targeted bacterial effectors, and on satellite RNAs

Highlights on #ribonucleotide salvage for #mitochondrial #transcription, and plant defense against #m6A-RNA-containing viruses

Further articles on #intraflagellar transport, #replication initiation within #chromatin, #integrin-dependent cancer cell invasion…

https://www.embopress.org/toc/14602075/2023/42/18

The EMBO JournalJul 5, 2023
Are there roles of DNA-incorporated N6-#methyldeoxyadenosine in animals?
Febrimarsa, Uri Frank et al #UniOfGalway show that its recycling from #m6A-containing RNA in #cnidarian embryos can delay #zygotic genome activation
https://www.embopress.org/doi/full/10.15252/embj.2022112934
The EMBO JournalJun 28, 2023

Is there N6-methyl-2’adenine DNA incorporation derived from decay of #m6A-RNA in cells?

Hailin Wang and colleagues show that mammalian ADAL deaminase sanitizes 2’-#deoxynucleotide pools to prevent AK1-dependent misincorporation

https://www.embopress.org/doi/10.15252/embj.2023113684