Here is an #article of mine that focused on #circulareconomy (doi: https://doi.org/10.1371/journal.pone.0290052). Liquid wastes generated from the leaf #protein extraction process from invasive plants was experimented on to test for the growth of #gutmicrobes capable of B12 production.
The hope was to make it into #probiotics / #postbiotics .
@academicchatter @academia_carnet #sustainability #foodproduction @amplisci
#GutMicrobes Played Role in #Evolution of #HumanBrain, New Study Suggests
https://www.sci.news/biology/gut-microbes-human-brain-evolution-14461.html
Newly Discovered #SixthSense Links #GutMicrobes to the #Brain in Real Time
https://medschool.duke.edu/stories/newly-discovered-sixth-sense-links-gut-microbes-brain-real-time
Diego V. Bohórquez, Ph.D, continues to redefine our understanding of gut-brain communication with new research uncovering a sixth sense that starts in the gut and may influence what we eat and how we feel.
A study found that gut bacteria diversity and specific microbial types are linked to mental health, influencing emotional responses, social interactions, thinking, and regulation. Greater microbial diversity generally supports better psychological functioning.
Autism could be diagnosed with stool sample, scientists say | Medical research | The Guardian
https://www.theguardian.com/science/article/2024/jul/08/autism-could-be-diagnosed-with-stool-sample-microbes-research
#Autism
#Diagnosis
#AutismDiagnosis
#GutMicrobes
#GutMicrobiome
#AutismGutMicrobiomeBiomarker
#Genetics
#StoolSamples
#Poo
#MetabolicPathways
Our physiology is complex and interconnected. Live dietary microbes (LDM) exert primary and secondary effects on our body.
For example, some studies show LDM have a primary positive effect on the respiratory system and a secondary effect on the gastrointestinal system, and vice versa.
A rich and varied diet inclusive of fermented foods may be critical to maintaining long term good health.
Check out the work: https://doi.org/10.1111/1750-3841.16893
Please have a look at our latest publication: https://doi.org/10.1371/journal.pone.0290052.
Producing leaf protein concentrates from Gorse generated a protein-depleted liquid by-product. We tried to valorise this fraction by growing vitamin B12 producing gut microbes.
#sustainability #circulareconomy #invasiveplants #gutmicrobes
Many commensal gut microbes are recognized for their potential to synthesize vitamin B12, offering a promising avenue to address deficiencies through probiotic supplementation. While bioinformatics tools aid in predicting B12 biosynthetic potential, empirical validation remains crucial to confirm production, identify cobalamin vitamers, and establish biosynthetic yields. This study investigates vitamin B12 production in three human colonic bacterial species: Anaerobutyricum hallii DSM 3353, Roseburia faecis DSM 16840, and Anaerostipes caccae DSM 14662, along with Propionibacterium freudenreichii DSM 4902 as a positive control. These strains were selected for their potential use as probiotics, based on speculated B12 production from prior bioinformatic analyses. Cultures were grown in M2GSC, chemically defined media (CDM), and Gorse extract medium (GEM). The composition of GEM was similar to CDM, except that the carbon and nitrogen sources were replaced with the protein-depleted liquid waste obtained after subjecting Gorse to a leaf protein extraction process. B12 yields were quantified using liquid chromatography with tandem mass spectrometry. The results suggested that the three butyrate-producing strains could indeed produce B12, although the yields were notably low and were detected only in the cell lysates. Furthermore, B12 production was higher in GEM compared to M2GSC medium. The positive control, P. freudenreichii DSM 4902 produced B12 at concentrations ranging from 7 ng mL−1 to 12 ng mL−1. Univariate-scaled Principal Component Analysis (PCA) of data from previous publications investigating B12 production in P. freudenreichii revealed that B12 yields diminished when the carbon source concentration was ≤30 g L−1. In conclusion, the protein-depleted wastes from the leaf protein extraction process from Gorse can be valorised as a viable substrate for culturing B12-producing colonic gut microbes. Furthermore, this is the first report attesting to the ability of A. hallii, R. faecis, and A. caccae to produce B12. However, these microbes seem unsuitable for industrial applications owing to low B12 yields.
#Fecal and #skin #microbiota of two rescued #Mediterranean #monkseal pups during #rehabilitation
https://journals.asm.org/doi/10.1128/spectrum.02805-23
@iucnredlist @iucnocean @iucn @redlist @IMPACTT @MicrobioJC #SciComm @academicchatter @academicsunite @openscience #gutmicrobes #hostmicrobiota #hostmicrobe #microbialecology #symbiosis #microbeinteractions @PeerJ
The AnimalAssociatedMetagenomeDB reveals a bias towards #livestock and developed countries and blind spots in functional-potential studies of #animal-associated #microbiomes
https://animalmicrobiome.biomedcentral.com/articles/10.1186/s42523-023-00267-3
#gutmicrobes #hostmicrobiota #hostmicrobe #microbialecology #symbiosis #microbeinteractions @MicrobioJC @PeerJ @IMPACTT @openscience @MicrobioJC @bioRxiv_microbio
Background Metagenomic data can shed light on animal-microbiome relationships and the functional potential of these communities. Over the past years, the generation of metagenomics data has increased exponentially, and so has the availability and reusability of data present in public repositories. However, identifying which datasets and associated metadata are available is not straightforward. We created the Animal-Associated Metagenome Metadata Database (AnimalAssociatedMetagenomeDB - AAMDB) to facilitate the identification and reuse of publicly available non-human, animal-associated metagenomic data, and metadata. Further, we used the AAMDB to (i) annotate common and scientific names of the species; (ii) determine the fraction of vertebrates and invertebrates; (iii) study their biogeography; and (iv) specify whether the animals were wild, pets, livestock or used for medical research. Results We manually selected metagenomes associated with non-human animals from SRA and MG-RAST. Next, we standardized and curated 51 metadata attributes (e.g., host, compartment, geographic coordinates, and country). The AAMDB version 1.0 contains 10,885 metagenomes associated with 165 different species from 65 different countries. From the collected metagenomes, 51.1% were recovered from animals associated with medical research or grown for human consumption (i.e., mice, rats, cattle, pigs, and poultry). Further, we observed an over-representation of animals collected in temperate regions (89.2%) and a lower representation of samples from the polar zones, with only 11 samples in total. The most common genus among invertebrate animals was Trichocerca (rotifers). Conclusion Our work may guide host species selection in novel animal-associated metagenome research, especially in biodiversity and conservation studies. The data available in our database will allow scientists to perform meta-analyses and test new hypotheses (e.g., host-specificity, strain heterogeneity, and biogeography of animal-associated metagenomes), leveraging existing data. The AAMDB WebApp is a user-friendly interface that is publicly available at https://webapp.ufz.de/aamdb/ .
Ajay Iyer
Steve Dustcircle 🌹
Biohacking Pathway
Steve Dustcircle 🌹
Punk Metal n Bowie
AutisticMumTo3
Kostas Kormas