Pipeline release! nf-core/smrnaseq v2.4.1 - v2.4.1 - 2026-01-07 - Navy Iron Boxer Patch!
A small-RNA sequencing analysis pipeline
Please see the changelog: https://github.com/nf-core/smrnaseq/releases/tag/2.4.1
#smallrna #smrnaseq #nfcore #openscience #nextflow #bioinformatics
Small RNA pathways can regulate eukaryotic antiviral defense. Here, the authors show that a member of the C. elegans dREAM complex regulates the antiviral RNAi pathway in response to Orsay virus infection, and if mutated it can generate a state of antiviral readiness even in the absence of virus.
Pipeline release! nf-core/smrnaseq v2.4.0 - v2.4.0 - 2024-10-14 - Gray Zinc Dalmation Patch!
Please see the changelog: https://github.com/nf-core/smrnaseq/releases/tag/2.4.0
#smallrna #smrnaseq #nfcore #openscience #nextflow #bioinformatics
Referenced link: https://phys.org/news/2023-06-scientists-small-rna-bacterial-infection.html
Discuss on https://discu.eu/q/https://phys.org/news/2023-06-scientists-small-rna-bacterial-infection.html
Originally posted by Phys.org / @physorg_com: http://nitter.platypush.tech/physorg_com/status/1669062165225938955#m
Scientists discover #smallRNA that regulates bacterial infection @georgiatech @nature https://www.nature.com/articles/s41586-023-06111-7 https://phys.org/news/2023-06-scientists-small-rna-bacterial-infection.html
People with weakened immune systems are at constant risk of infection. Pseudomonas aeruginosa, a common environmental bacterium, can colonize different body parts, such as the lungs, leading to persistent, chronic infections that can last a lifetime—a common occurrence in people with cystic fibrosis.
Tissue-specific overexpression of the double-stranded RNA transporter SID-1 limits lifespan in C. elegans
The small RNA landscape is stable with age in Drosophila
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0273590
Aging can be defined as the progressive loss of physiological homeostasis that leads to a decline in cellular and organismal function. In recent years, it has become clear that small RNA pathways play a role in aging and aging related phenotypes. Small RNA pathways regulate many important processes including development, cellular physiology, and innate immunity. The pathways illicit a form of posttranscriptional gene regulation that relies on small RNAs bound by the protein components of the RNA-induced silencing complexes (RISCs), which inhibit the expression of complementary RNAs. In Drosophila melanogaster, Argonaute 1 (Ago1) is the core RISC component in microRNA (miRNA) silencing, while Argonaute 2 (Ago2) is the core RISC component in small interfering RNA (siRNA) silencing. The expression of Ago1 and Ago2 is regulated by stress response transcription factor Forkhead box O (dFOXO) increasing siRNA silencing efficiency. dFOXO plays a role in multiple stress responses and regulates pathways important for longevity. Here we use a next-generation sequencing approach to determine the effects of aging on small RNA abundance and RISC loading in male and female Drosophila. In addition, we examine the impact of the loss of dFOXO on these processes. We find that the relative abundance of the majority of small RNAs does not change with age. Additionally, under normal growth conditions, the loss of dFOXO has little effect on the small RNA landscape. However, we observed that age affects loading into RISC for a small number of miRNAs.
nf-core
PLOS Biology
Phys.org
Bérénice A. Benayoun
Aging Highlights
Guido Puccetti