Nano-eradication of Recurrent Bladder Infections: Nanogel+penetrating agent+antibiotic kill bacteria that hide within bladder lining cells and perpetuate urinary tract infections.
#cystitis #bladder #infection #itaconate #nanogel #gentamycinhttps://www.instagram.com/reel/DHWrZMmM_b_/
Howard G. Smith MD, AM on Instagram: "Nano-eradication of Recurrent Bladder Infections
A nanogel coupled with a cell-penetrating compound more effectively delivers antibiotics to kill bacteria that hide within bladder lining cells and perpetuate urinary tract infections. Pharmacologists and microbiologists from the University of Colorado’s Anschutz Campus report the successful development and deployment of just such a drug.
The researchers combined polymeric nanogels with a peptide compound that eases their cellular entry and the antibiotic gentamicin in order to effectively kill pseudomonas bacteria that hide within bladder lining cells. When injected into the bladder, this nanogel-peptide-antibiotic complex is able to convey 36% more of the antibiotic into the cell interior than conventional administration techniques. When tested in animal models, it successfully eliminates more than 90% of bladder bacteria.
This technique shows great promise for more effective management of not only persistent and recurrrent bladder infections but also tenacious infections elsewhere in the urinary tract and other organs.
https://www.sciencedirect.com/science/article/abs/pii/S1549963425000127?via=ihub
https://www.sciencedaily.com/releases/2025/03/250312124140.htm#google_vignette
#cystitis #bladder #infection #itaconate #nanogel #gentamycin"
0 likes, 0 comments - drhowardsmithreports on March 18, 2025: "Nano-eradication of Recurrent Bladder Infections
A nanogel coupled with a cell-penetrating compound more effectively delivers antibiotics to kill bacteria that hide within bladder lining cells and perpetuate urinary tract infections. Pharmacologists and microbiologists from the University of Colorado’s Anschutz Campus report the successful development and deployment of just such a drug.
The researchers combined polymeric nanogels with a peptide compound that eases their cellular entry and the antibiotic gentamicin in order to effectively kill pseudomonas bacteria that hide within bladder lining cells. When injected into the bladder, this nanogel-peptide-antibiotic complex is able to convey 36% more of the antibiotic into the cell interior than conventional administration techniques. When tested in animal models, it successfully eliminates more than 90% of bladder bacteria.
This technique shows great promise for more effective management of not only persistent and recurrrent bladder infections but also tenacious infections elsewhere in the urinary tract and other organs.
https://www.sciencedirect.com/science/article/abs/pii/S1549963425000127?via=ihub
https://www.sciencedaily.com/releases/2025/03/250312124140.htm#google_vignette
#cystitis #bladder #infection #itaconate #nanogel #gentamycin".
New Treatment For Alopecia: Several compounds that, when swallowed and metabolized, create natural anti-inflammatory compounds that can help trigger the regrowth of hair.
#alopecia #itaconate #prodrug #baldness #steroids #minoxidil https://www.instagram.com/drhowardsmithreports/reel/DHWqt9XOIpA/
Howard G. Smith MD, AM on Instagram: "New Treatment For Alopecia
Chemists from the Czech Institute of Organic Chemistry and Biochemistry and the John’s Hopkins Drug Discovery unit have synthesized several compounds that, when swallowed and metabolized, create natural anti-inflammatory compounds that can help trigger the regrowth of hair. Alopecia areata is an autoimmune disease that produces hair loss and bald patches.
These newly-synthesized prodrug compounds, once within the body, lead to the production and release of itaconic acid and 1-methyl itaconate. Using a mouse model, the researchers show that oral administration of the prodrugs leads to release of these natural anti-inflammatory compounds with the skin. The prodrug to drug trick is necessary since the itaconate compounds themselves are not readily absorbed into the body.
This discovery is an exciting prospect for those suffering from alopecia. Results with conventional therapy using steroids, minoxidil, or, recently, the chemotherapeutic agent methotrexate have been unpredictable and fraught with undesirable side effects.. This itaconate prodrug treatment must still be refined for use in the clinic and undergo controlled trials, but, hopefully that will happen….someday soon.
https://pubs.acs.org/doi/10.1021/acs.jmedchem.4c02646
https://www.news-medical.net/news/20250313/Researchers-develop-new-compound-to-treat-alopecia-areata.aspx
#alopecia #itaconate #prodrug #baldness #steroids #minoxidil"
0 likes, 0 comments - drhowardsmithreports on March 18, 2025: "New Treatment For Alopecia
Chemists from the Czech Institute of Organic Chemistry and Biochemistry and the John’s Hopkins Drug Discovery unit have synthesized several compounds that, when swallowed and metabolized, create natural anti-inflammatory compounds that can help trigger the regrowth of hair. Alopecia areata is an autoimmune disease that produces hair loss and bald patches.
These newly-synthesized prodrug compounds, once within the body, lead to the production and release of itaconic acid and 1-methyl itaconate. Using a mouse model, the researchers show that oral administration of the prodrugs leads to release of these natural anti-inflammatory compounds with the skin. The prodrug to drug trick is necessary since the itaconate compounds themselves are not readily absorbed into the body.
This discovery is an exciting prospect for those suffering from alopecia. Results with conventional therapy using steroids, minoxidil, or, recently, the chemotherapeutic agent methotrexate have been unpredictable and fraught with undesirable side effects.. This itaconate prodrug treatment must still be refined for use in the clinic and undergo controlled trials, but, hopefully that will happen….someday soon.
https://pubs.acs.org/doi/10.1021/acs.jmedchem.4c02646
https://www.news-medical.net/news/20250313/Researchers-develop-new-compound-to-treat-alopecia-areata.aspx
#alopecia #itaconate #prodrug #baldness #steroids #minoxidil".
While
#targetedmetabolomics github repository is on private, we published a study where we identify
#itaconate, generated as a result of the metabolic rewiring during osteoclast differentiation. Kieler et al. Have a look!
https://shorturl.at/M64FI #multiomics #bioinformatics #biomedicine #meduniwien
Itaconate is a metabolic regulator of bone formation in homeostasis and arthritis
Objectives Bone remodelling is a highly dynamic process dependent on the precise coordination of osteoblasts and haematopoietic-cell derived osteoclasts. Changes in core metabolic pathways during osteoclastogenesis, however, are largely unexplored and it is unknown whether and how these processes are involved in bone homeostasis.
Methods We metabolically and transcriptionally profiled cells during osteoclast and osteoblast generation. Individual gene expression was characterised by quantitative PCR and western blot. Osteoblast function was assessed by Alizarin red staining. immunoresponsive gene 1 ( Irg1 ) - deficient mice were used in various inflammatory or non-inflammatory models of bone loss. Tissue gene expression was analysed by RNA in situ hybridisation.
Results We show that during differentiation preosteoclasts rearrange their tricarboxylic acid cycle, a process crucially depending on both glucose and glutamine. This rearrangement is characterised by the induction of Irg1 and production of itaconate, which accumulates intracellularly and extracellularly. While the IRG1–itaconate axis is dispensable for osteoclast generation in vitro and in vivo, we demonstrate that itaconate stimulates osteoblasts by accelerating osteogenic differentiation in both human and murine cells. This enhanced osteogenic differentiation is accompanied by reduced proliferation and altered metabolism. Additionally, supplementation of itaconate increases bone formation by boosting osteoblast activity in mice. Conversely, Irg1- deficient mice exhibit decreased bone mass and have reduced osteoproliferative lesions in experimental arthritis.
Conclusion In summary, we identify itaconate, generated as a result of the metabolic rewiring during osteoclast differentiation, as a previously unrecognised regulator of osteoblasts.
Data are available in a public, open access repository. Data are available upon reasonable request. Not applicable.
#Cortisone is highly effective at inhibiting the immune system – but how does it do it? A team from #CharitéBerlin & @FAU now shows @Nature that cortisone reprograms the metabolism of immune cells, activating a natural inflammatory “brake” present in the body. Based on their findings, the researchers now plan to develop anti-inflammatory treatments lower in side effects.
👉 https://www.charite.de/en/service/press_reports/artikel/detail/how_the_body_switches_out_of_fight_mode/
#CharitéPaper #CharitéImmunology #research #science #medicine #itaconate #rheumatism
Press reports
Press release of the Charité – Universitätsmedizin Berlin
Researchers have revealed the modulatory effect of the
#anti-inflammatory
#metabolite #itaconate on T helper and T regulatory
#cells, which may lead to new therapeutic approaches to treating some
#autoimmune #diseases.
#Medical #Biology #sflorghttps://www.sflorg.com/2023/03/med03152301.html
A common metabolite may help treat autoimmune diseases
Researchers have revealed the modulatory effect of the anti-inflammatory metabolite itaconate on T helper and T regulatory cells
Howard Smith MD, AM
Dr. Andrea Komljenovic 🇨🇭🇭🇷
Charité Berlin
Scientific Frontline