PARP1 (PDB: 4DQY) — catalytic core resolves NAD⁺ positioning (H133/E134/Mg²⁺) and substrate interface (326–332) as structurally separable sites. E134 as the ADP-ribose acceptor base.
Age-associated DNA damage → PARP1 hyperactivation → NAD⁺ depletion → SIRT1/3 inactivation → mitochondrial dysfunction. A structurally grounded feedforward loop in biological aging.
#PARP1 #NAD #StructuralBiology #LongevityBiology #Aging #DNARepair #Biogerontology #Sirtuins
🧬 Can controlling PARP1 turn CRISPR from a gene scissor into a gene sculptor?
🔗 Influence of PARP1 on CRISPR/Cas9 induced double strand break repair in proliferating cells. Computational and Structural Biotechnology Journal, DOI: https://doi.org/10.1016/j.csbj.2025.10.001
📚 CSBJ: https://www.csbj.org/
#CRISPR #GenomeEditing #Genomics #DNARepair #PARP1 #GeneticEngineering #MolecularBiology #GeneTherapy #CellBiology #SyntheticBiology #TherapeuticEditing
Making ends meet: Researchers find that a #protein superglue is crucial for #DNA #damage_repair.
https://phys.org/news/2024-02-protein-superglue-crucial-dna.html
Our DNA undergoes constant damage and repair. The most severe damage happens when the DNA breaks into two pieces, known as a double-strand DNA break. It creates two loose DNA ends that, if left unfixed, can lead to cell death. Researchers from the Biotechnology Center (BIOTEC) of the Dresden University of Technology have now answered the long-standing question of what keeps the broken DNA ends from being separated.
How a single #enzyme unleashes a complex #DNA #repair process.
https://phys.org/news/2023-04-enzyme-unleashes-complex-dna.html
Our DNA is constantly exposed to damaging factors that can lead to aging and age-related diseases such as cancer. PARP1 is an important enzyme that facilitates the response to DNA damage by attaching chemical groups called ADP-ribose to proteins. Published in Molecular Cell, a new study by the Matic group at the Max Planck Institute for the Biology of Ageing has discovered a second, slower wave of PARP1 activity that regulates DNA repair in a more gradual way.
Alice Frolov
Reprobio, biology reproduction
CSBJ
CellBioNews
oiger