Physical Mapping of 5S and 45S rDNA Sequences by FISH in Species of the Genus Iris L. - Cytology and Genetics

Abstract For the first time, FISH was used to map 5S and 45S rDNA sequences in two species of the genus Iris L. Ten major 45S rDNA sites were detected on chromosomes and interphase nuclei of Iris pumila L. and six sites in Iris pseudopumila Tineo; 12 loci 5S rDNA were found in I. pumila, while only two were in I. pseudopumila. A high level of aneuploidy (60–80%) was revealed in I. pumila plants, which is probably associated with the hybrid origin of the species. Given the obtained results, it is quite probable that I. pseudopumila may be one of the parental forms of the allotetraploid I. pumila. The obtained data are the basis for further evolutionary studies of this species. The evolutionary changes of diploid ancestral forms following the formation of allopolyploids and the importance of ribosomal DNA sequences in detecting these changes are discussed.

INDEGSAL-ULe (@[email protected])

0 Posts, 2 Following, 0 Followers · Instituto de Sanidad Animal y Desarrollo Ganadero, Univ. de León. Investigación en sanidad, producción y reproducción animal. Servicios al sector ganadero, clínico e industrial. Docencia. Institute for Animal Health and Livestock Development, University of León, Spain. Research in animal health, production, and reproduction. Services to livestock breeders, industry, and veterinary clinical sector. OneHealth approach. Teaching. #AnimalHealth #OneHealth #AnimalProduction #Livestock #HigherEd

INDEGSAL-ULe (@[email protected])

0 Posts, 2 Following, 0 Followers · Instituto de Sanidad Animal y Desarrollo Ganadero, Univ. de León. Investigación en sanidad, producción y reproducción animal. Servicios al sector ganadero, clínico e industrial. Docencia. Institute for Animal Health and Livestock Development, University of León, Spain. Research in animal health, production, and reproduction. Services to livestock breeders, industry, and veterinary clinical sector. OneHealth approach. Teaching. #AnimalHealth #OneHealth #AnimalProduction #Livestock #HigherEd

HIF1A contributes to the survival of aneuploid and mosaic pre-implantation embryos - preLights

Hypoxia regulates embryonic fitness in mosaic aneuploid embryos

Impact of Different DNA Ploidy Patterns on Endometrial Carcinomas Based on Image Cytometry - Cytology and Genetics

Worldwide, endometrial cancer is one of the most frequently diagnosed malignancies in women and a notable cause of death. The aim of this study was to perform image cytometric DNA ploidy analysis on a prospective material of endometrial adenocarcinomas in order to determine potential correlation between ploidy status and their histological features. The analysis was carried out in fresh tissue samples resected by implementing complete hysterectomy in a series of patients (n = 126). We found that ploidy status using image cytometry correlate with histologic type, grade and stage in endometrial cancer and aneuploid tumor samples are associated with aggressive phenotype statistics. Furthermore, DNA ploidy should be used as a reliable and applicable prognostic marker in the routine clinical practice.

Press reports

Press release of the Charité – Universitätsmedizin Berlin

CRISPR gene editing - Wikipedia

Effects of aneuploidy on cell behaviour and function - Nature Reviews Molecular Cell Biology

Aneuploidy affects organisms from early development through to aging and is a cause of pregnancy loss and cancer. Recent studies have increased our understanding of its mechanisms and how it can be both beneficial and detrimental to cells and organisms, depending on the karyotype and external cues. These insights shed light on its roles in human pathogenesis and on genome evolution.