Pipeline release! nf-core/scrnaseq v4.0.0 - 4.0.0!

Please see the changelog: https://github.com/nf-core/scrnaseq/releases/tag/4.0.0

#10xgenomics #10xgenomics #alevin #bustools #cellranger #kallisto #rnaseq #singlecell #starsolo #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/scrnaseq v3.0.0 - 3.0.0!

Please see the changelog: https://github.com/nf-core/scrnaseq/releases/tag/3.0.0

#10xgenomics #10xgenomics #alevin #bustools #cellranger #kallisto #rnaseq #singlecell #starsolo #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/scrnaseq v2.7.1 - 2.7.1!

Please see the changelog: https://github.com/nf-core/scrnaseq/releases/tag/2.7.1

#10x-genomics #10xgenomics #alevin #bustools #cellranger #kallisto #rna-seq #single-cell #star-solo #nfcore #openscience #nextflow #bioinformatics

Pipeline release! nf-core/scrnaseq v2.7.0 - 2.7.0!

Please see the changelog: https://github.com/nf-core/scrnaseq/releases/tag/2.7.0

#10x-genomics #10xgenomics #alevin #bustools #cellranger #kallisto #rna-seq #single-cell #star-solo #nfcore #openscience #nextflow #bioinformatics

I'm very happy to announce that MultiQC v1.15 was just released! 🎉

https://github.com/ewels/MultiQC/releases/tag/v1.15

It comes with 🐛 fixes for #10xGenomics #CellRanger fix, #mosdepth tweaks, and supports a new tool #Librarian to predict library type from FastQ base composition 🕵🏻‍♂️ 🧬plus much more!

Also included is a ~5-7 fold speed-up in the initial file search. This will shave off a chunk of run-time off for people generating big reports 🏎💨

Thanks to Vlad Savelyev for this with a hat tip to Pontus Höjer at the end 🙏🏻

Split a #BAM file produced by #CellRanger into one file per #singlecell barcode, using #samtools and #awk:

samtools view in.bam | awk '/CB:Z:/{f=substr($0,match($0,/CB:Z:[ACGT]{16}/)+5,16)".txt"}{print>>f}'