Development of a multi-species #luciferase-based double #antigen #ELISA for the detection of #antibodies against #Influenza A virus #H5 clade 2.3.4.4b, https://etidiohnew.blogspot.com/2026/01/development-of-multi-species-luciferase.html
Development of a multi-species #luciferase-based double #antigen #ELISA for the detection of #antibodies against #Influenza A virus #H5 clade 2.3.4.4b

#Warning is not spam! Don't be so aggressively indifferent! #PCR #tests and #vaccinations contain #Luciferase - PCR is used to #biohack your #DNA, you can request removal!
Dr. Eric Schreiter demo at @hhmijanelia #bioluminescence #luciferase
#Luciferase-Assays sind Standard in vielen Zell-Labors, die kommerziellen Kits sind jedoch keine Schnäppchen. Andrea Pitzschke beschreibt, wie man mit einem hauseigenem Lysepuffer und simplen Modifikationen die Assay-Kosten drosseln kann: https://laborjournal.de/editorials/3023.php #Labor #LaborTool
Günstiger Luciferase-Assay

iger ist ...

Laborjournal
A chemically defined, xeno-free culture system for culturing and deriving monkey pluripotent stem cells in vitro

Non-human primates (NHPs) have a high degree of similarity to humans compared to other animal models. These similarities manifest at the genetic, physiological, socio-behavioral, and central nervous system levels, making NHPs uniquely suitable for research into stem cell therapy and increasingly used in preclinical trials to test the safety and efficacy of biotechnology therapies.

Phys.org
Simplifying the way cell proliferation is monitored in mice

Observing cell proliferation in living animals for a long period requires collecting and analyzing animal organs at multiple points in time. This cumbersome process requires an abundance of resources, including animals.

Phys.org

@PhiloNeuroScie

“To expand the repertoire of endogenous cellular communication, we developed a synthetic, photon-assisted synaptic transmission (PhAST) system. PhAST is based on luciferases and channelrhodopsins that enable the transmission of a neuronal state across space, using photons as neurotransmitters.”

Porta-de-la-Riva et al. 2023 https://www.nature.com/articles/s41592-023-01836-9

What a fun project and cool finding, and made in #Catalunya to top it off at the Photonic Science Institute.

Combine that with #photopharmacology and neural circuits may be able to dose themselves according to activity patterns.

#neuroscience #PhAST #Luciferase #ChannelRhodopsin #biophotonics #optogenetics

Neural engineering with photons as synaptic transmitters - Nature Methods

PhAST is a technology for establishing de novo or modulating synaptic transmission in a light-dependent manner in C. elegans. By combining a calcium-dependent luciferase on pre-synapses with channelrhodopsin on post-synapses, light serves as a synthetic neurotransmitter.

Nature

I have created a little R #Shiny interface to deconvolute #luciferase assay data.

This is based on Brown 2008 - Inferring gene expression dynamics from reporter protein levels (but does not include volume correction)

The app also reports initial rate, maximum and decay rate

It is available here, for anyone to enjoy, modify etc

https://github.com/nicolaromano/LucDeconvolution

#rstats #analysis #biology #transcription

GitHub - nicolaromano/LucDeconvolution: A simple Shiny app to deconvolute luciferase assay data

A simple Shiny app to deconvolute luciferase assay data - GitHub - nicolaromano/LucDeconvolution: A simple Shiny app to deconvolute luciferase assay data

GitHub

Tuning plant #promoters using a simple split #luciferase method for quantifying transcription factor binding affinity | bioRxiv

https://www.biorxiv.org/content/10.1101/2023.02.13.528283v1

Things to do #InsteadOfWorldCup, 20/29:

#Molecules — who doesn’t love ‘em? With the linked tool #MolView, you can visualize them in all sorts of ways and even create your own.

Here’s a structural image of #luciferase, which (if you can somehow manage to synthesize it in your buttocks) will make your arse glow in the dark.

Boost and add your own!

#NoWorldCup2022 #Boycott #FIFA #Qatar #CorruptWretches #ContraIlCalcioModerno #SCIENCE! #GlowInTheDarkArsesFTW

https://molview.org

MolView

MolView