A chemically defined, #xeno_free culture system for #culturing and deriving #monkey #pluripotent stem cells in vitro.
#bioengineering #signalling #luciferase #chimera
https://phys.org/news/2023-12-chemically-xeno-free-culture-culturing-deriving.html
Non-human primates (NHPs) have a high degree of similarity to humans compared to other animal models. These similarities manifest at the genetic, physiological, socio-behavioral, and central nervous system levels, making NHPs uniquely suitable for research into stem cell therapy and increasingly used in preclinical trials to test the safety and efficacy of biotechnology therapies.
Simplifying the way #cell #proliferation is monitored in #mice.
“To expand the repertoire of endogenous cellular communication, we developed a synthetic, photon-assisted synaptic transmission (PhAST) system. PhAST is based on luciferases and channelrhodopsins that enable the transmission of a neuronal state across space, using photons as neurotransmitters.”
Porta-de-la-Riva et al. 2023 https://www.nature.com/articles/s41592-023-01836-9
What a fun project and cool finding, and made in #Catalunya to top it off at the Photonic Science Institute.
Combine that with #photopharmacology and neural circuits may be able to dose themselves according to activity patterns.
#neuroscience #PhAST #Luciferase #ChannelRhodopsin #biophotonics #optogenetics
PhAST is a technology for establishing de novo or modulating synaptic transmission in a light-dependent manner in C. elegans. By combining a calcium-dependent luciferase on pre-synapses with channelrhodopsin on post-synapses, light serves as a synthetic neurotransmitter.
I have created a little R #Shiny interface to deconvolute #luciferase assay data.
This is based on Brown 2008 - Inferring gene expression dynamics from reporter protein levels (but does not include volume correction)
The app also reports initial rate, maximum and decay rate
It is available here, for anyone to enjoy, modify etc
Tuning plant #promoters using a simple split #luciferase method for quantifying transcription factor binding affinity | bioRxiv
Things to do #InsteadOfWorldCup, 20/29:
#Molecules — who doesn’t love ‘em? With the linked tool #MolView, you can visualize them in all sorts of ways and even create your own.
Here’s a structural image of #luciferase, which (if you can somehow manage to synthesize it in your buttocks) will make your arse glow in the dark.
Boost and add your own!
#NoWorldCup2022 #Boycott #FIFA #Qatar #CorruptWretches #ContraIlCalcioModerno #SCIENCE! #GlowInTheDarkArsesFTW