`We find that combining RNA-seq perturbation-based networks with VIPER (Virtual Inference of Protein Activity by Enriched Regulon analysis) accurately captures splicing factor activity as modulated by multiple regulatory layers. This approach integrates splicing factor regulation into a single score derived solely from exon inclusion signatures, allowing functional interpretation of heterogeneous conditions.`

https://doi.org/10.1038/s41467-026-69642-3

#splicing #RNA #genetics #exon #assay

#biology #assay cell migration

Original post: https://bsky.app/profile/did:plc:rxlho3dvaknfvlnbzgovjzfk/post/3mabafm6wfc2b

Rapid #neutralizing #assay for circulating #H5N1 #influenza virus in dairy #cows, BioRxIV: https://www.biorxiv.org/content/10.1101/2024.07.30.605731v1

Our results suggest that the circulating H5N1 virus in cows shares #antigenic characteristics with NIID-002, providing significant implications for the #development and preparation of #vaccines.

Profiling endogenous #airway #proteases and antiproteases and measuring proteolytic #activation of #Influenza #HA using in vitro and ex vivo human airway surface #liquid samples, BioRxIV: https://www.biorxiv.org/content/10.1101/2024.06.14.599031v1?rss=1

This #assay presents a direct and efficient method of evaluating the proteolytic environment of human airway samples in assessment of therapeutic treatment, exposure, or underlying disease.

Enzyme Immunoassays (EIA) 

#Assay, #EIA, #Heterogeneous, #Homogenous #Immunology Enzymes labelled conjugates were first introduced in 1966 for localization of antigens in tissues, as an alternative to fluorescent conjugates. …. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -

https://micrordt.wordpress.com/2024/04/23/enzyme-immunoassays-eia/

Radioimmunoassay (RIA) 

#Antibody, #Antigen, #Assay, #RadioAntigen, #RIA #Immunology Besides fluorescent dyes, many other distinctive ‘labels’ also can be conjugated to antigen and antibodies. The most commonly used labels…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -

https://micrordt.wordpress.com/2024/04/23/radioimmunoassay-ria/

Detection Of Specific Antigen By Using Enzyme-Linked Immuno-Sorbent Assay (ELISA) Technique

#Agglutination, #Assay, #ELISA, #EnzymeLinked, #Technique #Biotechnology, #Immunology The ELISA is an immunological technique which is used to measure either antigen or antibodies present in serum. For the…. Medical Microbiology & RDT Labs | Read More -

https://micrordt.wordpress.com/2024/04/19/detection-of-specific-antigen-by-using-enzyme-linked-immuno-sorbent-assay-elisa-technique/