`We find that combining RNA-seq perturbation-based networks with VIPER (Virtual Inference of Protein Activity by Enriched Regulon analysis) accurately captures splicing factor activity as modulated by multiple regulatory layers. This approach integrates splicing factor regulation into a single score derived solely from exon inclusion signatures, allowing functional interpretation of heterogeneous conditions.`
Alternative transcription initiation, splicing and polyadenylation generate extensive transcript diversity in eukaryotes, but its evolutionary significance has been disputed. This study analyses 166 transcriptomes across 75 metazoan species to show that transcript diversity generally decreases with effective population size, supporting the view that most transcript diversity reflects deleterious RNA processing errors rather than adaptive functions.
Xinlai Cheng gave an interesting talk at #Biochemistry2026. He talked about his group's research into "Targeting the Spliceosomal Protein USP39 Through Allosteric Ligands and PROTAC-Induced Degradation".
https://onlinelibrary.wiley.com/doi/10.1002/anie.202516809
#Splicing #Biochemistry #Chemistry #ChemBio
CC: @gdch
Alternativní splicing (Genetika 🧬)
Alternativní splicing je jev, při němž při transkripci vzniká z jednoho genu více variant mRNA potenciálně kódujících rozdílné bílkovinné produkty. Po přepisu DNA v jádře prochází tzv. primární transkript RNA úpravami, v jejichž rámci se dlouhé nekódující úseky vystřihují a ostatní sekvence je možné spojit různými způsoby. Z výsledných několika RNA vytvořených od...
PLOS Biology
Big Fl👀f is watching you.
katch wreck
GDCh Division of Biochemistry
Paul SomeoneElse
T_X
Planetární ekolog
Sustainable Sailing
JIPB