I spend amazing two days at the #pristionchus conference in Tübingen. Psyched by a lot of good conversations, I can’t wait to continue with my research on the feeding decision in P. pacificus. To everyone looking for a great and welcoming scientific community to study #evodevoneuro, as Curtis Loer put it, or the #newnaturalhistory as Mary Jane West-Eberhard did, the Pristionchus crowd might just be the right fit!
#MastodonScience #nematode #decisionmaking #neuroscience #neurobiology
@manlius Yes, a lot, but generated mostly with #CATMAID which is more purpose-built for #connectomics.
An early reconstruction of a neural circuit done with #TrakEM2 was by Davi Bock et al. 2011 on the mouse visual cortex, "Network anatomy and in vivo physiology of visual cortical neurons" https://www.nature.com/articles/nature09802
Another one with #TrakEM2 was by Dan Bumbarger et al. 2013 "System-wide rewiring underlies behavioral differences in predatory and bacterial-feeding nematodes" where they compared #celegans with another nematode, #pristionchus pacificus that has the exact same amount of neurons but connected differently https://www.sciencedirect.com/science/article/pii/S0092867412015000
Later ones with #CATMAID include:
The polychaete worm #Platynereis by @jekely 's group, "Whole-animal #connectome and cell-type complement of the three-segmented Platynereis dumerilii larva" Verazto et al. 2020 https://www.biorxiv.org/content/10.1101/2020.08.21.260984v2.abstract
And all of ours in #Drosophila larva. See the #VirtualFlyBrain server which hosts the #vEM of the whole central nervous system and lists all the neurons included in each published paper (currently 23), shared among the papers and all connecting to each other: https://l1em.catmaid.virtualflybrain.org/?pid=1&zp=108250&yp=82961.59999999999&xp=54210.799999999996&tool=tracingtool&sid0=1&s0=2.4999999999999996&help=true&layout=h(XY,%20%7B%20type:%20%22neuron-search%22,%20id:%20%22neuron-search-1%22,%20options:%20%7B%22annotation-name%22:%20%22papers%22%7D%7D,%200.6)
The 24th will come soon, featuring the complete whole #Drosophila larval brain with ~2,500 neurons. It's under review.
To date, various aspects of connectivity have been inferred from electron microscopy (EM) of synaptic contacts, light microscopy of axonal and dendritic arbors, and correlations in activity. However, until now it has not been possible to relate the complex structural wiring between neurons to the function of individual cells. Using a combination of functional imaging and three-dimensional serial EM reconstruction at unprecedented scale, two papers now describe the connectivity of single cells in the mouse visual system. This study investigates the connectivity of inhibitory interneurons in primary visual cortex.
@leoboeger The famous #pristionchus! Proud to have helped Dan Bumbarger [1] back in Zurich in 2010 or so when he visited me to learn how to register his serial sections with #TrakEM2. It’s been a while.
[1] Bumbarger et al. 2013 https://www.sciencedirect.com/science/article/pii/S0092867412015000
Leonard Böger
Albert Cardona
leoboeger