#Investigation and #impact of mammalian #adaptation markers on #H5N8 high pathogenicity avian #influenza #polymerase activity

Mechanism of co-transcriptional cap snatching by #influenza #polymerase

#Polymerase #mutations underlie early #adaptation of #H5N1 #influenza virus to dairy #cattle and other #mammals

Emergence of #mammalian-adaptive #PB2 #mutations enhances #polymerase activity and #pathogenicity of #cattle-derived #H5N1 #influenza A virus

An evolutionary approach to identify #mammalian adaptive #mutations in the avian #influenza #polymerase complex

#Polymerase #mutations underlie early #adaptation of #H5N1 #influenza virus to dairy #cattle and other #mammals.

A portable low-cost #Arduino-controlled #OpenSource #polymerase chain reaction device:

-4-well aluminum heating block
-heated lid
-heating/cooling rates: 1.78/1.52 °C/s
- temp. accuracy: ± 0.55 °C
-cost: US $120
-requires #MATLAB

https://doi.org/10.1016/j.ohx.2025.e00635
#DIYbio #lab #instruments #PCR #DNA

#Influenza A virus #polymerase co-opts distinct sets of host proteins for #RNA transcription or #replication

#Polymerase #mutations underlie #adaptation of #H5N1 #influenza virus to dairy #cattle and other #mammals.