Aquarius helicase facilitates HIV-1 integration into R-loop enriched genomic regions.

#HIV1 #HIV1Integration #Rloops #Integrase #Helicases #IntronBindingComplex

https://www.nature.com/articles/s41564-025-02089-2

More #phage curiosities - two closely related phages likely have their #integrase at a particular location (based on clustering, alignment of related, better studied phages and hmm screening) - but they're split into a couple of pieces.

Are there examples of split/mulit-part integrase still functioning in nature? Or are we looking at #sequencing (illumina shorts) error? Hmm.

#microbiology #bioinformatics

Characterization of #Integrase and #Excisionase Activity in a #Cell-Free #Protein Expression System Using a Modeling and Analysis Pipeline | #synbio

https://pubs.acs.org/doi/10.1021/acssynbio.2c00534

Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases

https://www.nature.com/articles/s41587-022-01527-4

#crispr #integrase #GenomeEngineering #synbio