Seth Howes (@SethSHowes)

집에서 주방 테이블 위에서 직접 유전체를 시퀀싱한 경험과, 사용한 장비·프로토콜·이론·비용까지 상세히 정리했다고 밝혔다. 개인용 바이오/AI 분석과 결합될 수 있는 DIY 유전체 시퀀싱 사례로, 실험 자동화와 데이터 분석 응용 가능성을 시사한다.

https://x.com/SethSHowes/status/2045782975380406623

#genomics #diybio #sequencing #bioinformatics

Single-molecule #peptide #sequencing through reverse translation of peptides into DNA
https://www.nature.com/articles/s41587-026-03061-z

everything that can be bound with/converted to DNA can be "sequenced": it's sort of like SBS, but here DNA oligos are used instead of individual DNA bases and later actual SBS is performed to decode each oligo sequence... every read will inform about single aa at single position from single protein.
to sequence a protein with 300 aa, you'll need to sequence at least 300 reads!
neat!

RE: https://genomic.social/@jfy133/116091864495660495

🚨 LAST CHANCE! 🚨

🧬 Do you work with #sequencing data, and have a spare 5 minutes to help contribute to making #genomics #metadata standards more #FAIR?|

🎰 Want a chance to win a little prize pack?

💡 Reminder of the our @NFDI4Microbiota and Genomic Standards Consortium funded survey (link below)!

📅 Deadline is 20th March (end of tomorrow!)!

How can #epigenetic modifications of #DNA be studied to better understand #biology?

In her talk at #Biochemistry2026, Sabine Schneider talked about her group's exciting work on developing new TET3 enzymes that improve epigenetic sequencing.

https://pubs.rsc.org/en/content/articlelanding/2025/cb/d4cb00315b
#Chemistry #Biochemistry #ChemBio #Sequencing
CC: @gdch, @GDCh_BioChem

Interesting talk by Sabine Schneider at #Biochemistry2026. She talked about her group's work on "Novel TET3 enzymes for next-generation epigenetic sequencing".

https://pubs.rsc.org/en/content/articlelanding/2025/cb/d4cb00315b
#Epigenetics #Biochemistry #ChemBio #Chemistry #Sequencing #TET
CC: @gdch

RE: https://genomic.social/@jfy133/116091864495660495

😤 Do you work with #sequencing data, and find it frustrating to fill in #metadata to make your data more #FAIR ?

💡 Reminder of the survey on #genomics metadata standards below!
📅 Deadline on the 20th March!
🎰 We have a raffle to win a little prize pack

#Linux tools for examining #sequencing data:

```
zcat sequences.fastq.gz | \
awk 'NR % 4 == 2 { print substr($0, 0, 10) }' |\
sort | uniq -c | sort -nr > index_list
```

Generates a list of the first 10 bases in sequences.fastq.gz, sorted by frequency. Handy if you need to check that the indexes you thought you used are in fact the ones that were used.

```
$ head index_list
10765621 ACGTACTGTG
9006389 AACCACGCTT
6891089 ACACCGTTGC
5198342 AGAAGCCATT
4202651 AATCGAGGTG
3788448 ACCTTGTGCA
3271929 ACGAGTTGCA
3027755 ACCGATTGCA
2682797 ACTATCCGTG
54372 ACGTANTGTG
...
```

This may become a blogpost at some point.

#bioinformatics