How would a signal look like from our micro-ring resonator #MRR #biosensor?

We observe a spectrum in a wavelength range around 1.55 µm by coupling the laser radiation into an array of five MRRs, see bottom figure. It is notable that each MRR has a curved triangular shape of different size this causes the resonance peak of each MRR to occur at different positions in the spectrum.

In the spectrum range investigated, we observed four groups of resonance peaks corresponding to four standing waves occurring to each of the five rings.

Determining the perimeter of the ring was challenging. I used #ImageJ to draw a polygon that approximated the triangular shape and measured the perimeter. Then, with the use of the formulas obtained from P. Steglich, et al(https://ieeexplore.ieee.org/abstract/document/9568878), it was possible to determine the effective refractive index nff and the order m of the standing wave.

#microelectronic #bioelectronic

@stephenturner.us

This is great spatially-resolved transcriptomics by the R/Bioconductor package visiumStitched, which facilitates stitching the images together with Fiji (ImageJ). 🦾

#rstats #Fiji #ImageJ #Bioconductor #visiumStitched

Bin heute mit den Automatismen von #ImageJ / #Fiji nicht vollends zufrieden gewesen. Wahrscheinlich sind meine Partikel zu klein.
Jetzt habe ich das manuell erledigt.

Hier lohnt sich mal wieder die Stifteingabe.

Habe insgesamt über 300 Partikel analysiert, das genügt für die grobe Statistik und ich kann zeigen, dass die so grob 10 nm Durchmesser haben.
#MöphDiss

Edit: Das zweite Bild ist nur ein Zwischenstand.

Tried the same with a more realistic 3D stack from the #ImageJ sample library.#Cellpose runs fast and segments very well out of the box.#CellSeg3D takes considerably longer and seems to segment decently, but I couldn’t get a proper instance #segmentation in the post-processing step (which is recommended as part of its workflow). However, #CellSeg3D looks very promising — just needs some more time and parameter exploration, I guess.

I’d recommend giving it a try 👌