white=nucleus staining
blue=cell staining

why does it feel like the nucleus in the middle decided to live outside its own cell body?

#ImageAnalysis

🔬 Registration is open for our pilot Introduction to napari Workshop!

napari is a powerful open-source image viewer for scientific data analysis in Python. This hands-on workshop will get you exploring multi-dimensional datasets fast.

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Two workshops at two different times.

#napari #Python #ImageAnalysis #DataScience #OpenSource

[Show GN: AI 이미지 스타일 코치 (연예인 닮은꼴)

AI 이미지 스타일 코치 서비스인 'Show GN'이 사진 한 장을 분석하여 사용자의 분위기와 이미지에 맞는 스타일을 추천해주는 서비스입니다. 연예인 닮은꼴 비교가 아닌, 분위기 기반의 스타일 코칭을 제공합니다.

https://news.hada.io/topic?id=26147

#ai #style #recommendation #fashion #imageanalysis

What would you align sets of multiple (~20) large (2-4 Gb) #microscopy images?

For smaller subset images ImageJ plugins for transformations based on SIFT landmark correspondence work well. However standard ImageJ (bioformats) file handling doesn’t cope well with such large files. For plugins handling large file manipulation (BigData family) or chunked (e.g. zarr) storage in turn I don’t know how to implement SIFT (or similar) - e.g. for BigWarp I can only find manual landmark annotation, i.e. no option to create landmarks via other plugins.

My images are iterative fluorescence whole slide scans of the same slide with a constant nuclear stain and varying other stains. There is some x/y shift and rotation as well as warping - nothing major, but I need nearly pixel perfect alignment (e.g. QuPath+Warpy worked well on larger images but was too imprecise).
Stitching happens on the fly during imaging and I’m not sure I can extract the tiles faithfully, so the ASHLAR pipeline didn’t seem applicable. I’ve seen VALIS recommended, but implementation seemed daunting and since the nuclear stain provides reasonable fiducial points the workflow seemed an overkill.

Ideally I would want a scripted solution as this has to scale up to hundreds of such sets eventually and downstream processing is in python+R anyhow.

#imageanalysis #spatial #imaging

I have two open positions in my lab at the Advanced Light Microscopy Unit Centre for Genomic Regulation (CRG):

- Imaging Scientist (permanent position. Deadline 11th Nov.) https://recruitment.crg.eu/content/jobs/position/imaging-scientists-advanced-light-microscopy-unit-almu

- Entry-Level Imaging Scientist (12 months fixed-term position. Deadline 18th Nov.) https://recruitment.crg.eu/content/jobs/position/entry-level-imaging-scientist-advanced-light-microscopy-unit-almu

If you have any questions don’t hesitate to reach out.

Boosts appreciated.

#getfedihired #fedihire #jobSearch #jobposting #Microscopy #Optics #ImageAnalysis

Position open: Head of the Center for Microscopy and Image Analysis (Core Facility) at the Center for Microscopy and Image Analysis (ZMB) in the University of Zurich, Switzerland.

https://www.nature.com/naturecareers/job/12847076/head-of-the-center-for-microscopy-and-image-analysis-core-facility-/

#microscopy #PhDJobs #academia #ElectronMicroscopy #LightMicroscopy #ImageAnalysis