Pablo J. Sáez 

@[email protected]
422 Followers
106 Following
140 Posts
Husband - Dad of 2 - Scientist 🇨🇱 Chilean expat / #NewPI @cellcommlab
/ I love to see how cells walk & talk
#Microscopy #CellMigration #CellComm #CalciumSignaling #Organelles #IonChannels
Google Scholarhttps://scholar.google.com/citations?user=3qgZ6x4AAAAJ&hl=en
CellComM Labhttps://www.uke.de/kliniken-institute/institute/biochemie-und-molekulare-zellbiologie/forschung/arbeitsgruppen/cell-communication-and-migration-laboratory.html
Pablo J. Sáez May 4, 2023

#MayThe4th & #CellMigration

#DarthVader cell using the force to choke another one (arrow/red nucleus) until...well, wait for it to see it in slow motion
Only the sound is missing to be like #StarWars

#scicomm @cellcommlab @focalplane_jcs #MayThe4thBeWithYou #cell

Pablo J. Sáez Apr 30, 2023

For the long weekend.

#Mitochondria, powerhouse of the #cell were labeled (cyan) and it's possible to visualize how they reach every corner.

All recorded under the microscope by @KuxJohan & @Marie_Adler23 @cellcommlab

#scicomm #microscopy @focalplane_jcs @Mito_News @MitoAging

Pablo J. Sáez Apr 28, 2023
Last week @Martina_Alamo_R finished her internship @ the @cellcommlab, and left Hamburg.
Now she is back home @LabYuseff 🇨🇱 to finish her #PhD thesis.
Good luck Marti, we already miss you (and your experiments of 300 coverslips 😅 support pic 👇🏼)
Pablo J. Sáez Apr 25, 2023

Hi tweeps do you know any free/good/fast/easy to use screen recorder?

We were using https://www.freescreenrecording.com/ before they decided to mess up everything and ask for money.

With @JBoixCampos we tried several others but nothing worked like that one...

#askingforafriend

Free Cam — Free Screen Recording & Video Editing Software

Easy screen recorder for Windows. A complete set of functions for creating, editing and instantly sharing high-quality screencasts. 100% free for life!

Pablo J. Sáez Apr 17, 2023
Unboxing Monday.
First things to install @FijiSc @ilastik_team @CellProfiler.
To better analyse cell shape, #cellmigration and much more.
Show threadPablo J. Sáez Apr 14, 2023

Here the result of the 1st test

---
RT @pj_saez
For a late #FluorescenceFriday

Expansion microscopy + SRFF = #ExSRFF

By @PuellesVictor lab and co.
https://www.nature.com/articles/s41565-023-01328-z #scicomm @focalplane_jcs https://twitter.com/pj_saez/status/1645885421367316482
https://twitter.com/pj_saez/status/1646990848419065861

Expansion-enhanced super-resolution radial fluctuations enable nanoscale molecular profiling of pathology specimens - Nature Nanotechnology

Complex clinical samples can be imaged with 25 nm resolution using a light-emitting-diode-based wide-field microscope.

Nature
Pablo J. Sáez Apr 14, 2023

For a late #FluorescenceFriday

Expansion microscopy + SRFF = #ExSRFF

By @PuellesVictor lab and co.
https://www.nature.com/articles/s41565-023-01328-z #scicomm @focalplane_jcs
---
RT @pj_saez
#ExSRRF, a new pipeline that allows to reach 25 nm resolution using widefield #microscopy
(useful for #cellbio, #ER_literature, clinics & more)

Tks for the invitation, @PuellesVictor, happy that @cellcommlab we could help you w this neat work

@Na…
https://twitter.com/pj_saez/status/1645885421367316482

Expansion-enhanced super-resolution radial fluctuations enable nanoscale molecular profiling of pathology specimens - Nature Nanotechnology

Complex clinical samples can be imaged with 25 nm resolution using a light-emitting-diode-based wide-field microscope.

Nature
Pablo J. Sáez Apr 14, 2023

Hi tweeps, is it possible to create an image with a sliding bar using @FijiSc ?
(If not, which tool do you use?)

I mean images like this beautiful one from @Abberior
https://abberior.rocks/superresolution-confocal-systems/stedycon/
Maybe I can get a hint from @HenriquesLab @christlet
#askingforafriend

STEDYCON - @abberior.rocks

Just upgrade your existing widefield system to a confocal and STED microscope with a resolution down to 30 nm. Enjoy fine imaging at the push of a button!

@abberior.rocks
Pablo J. Sáez Apr 11, 2023

#ExSRRF, a new pipeline that allows to reach 25 nm resolution using widefield #microscopy
(useful for #cellbio, #ER_literature, clinics & more)

Tks for the invitation, @PuellesVictor, happy that @cellcommlab we could help you w this neat work

@NatureNano https://www.nature.com/articles/s41565-023-01328-z

Expansion-enhanced super-resolution radial fluctuations enable nanoscale molecular profiling of pathology specimens - Nature Nanotechnology

Complex clinical samples can be imaged with 25 nm resolution using a light-emitting-diode-based wide-field microscope.

Nature
Pablo J. Sáez Apr 10, 2023

Nematics of collective cell migration @ #monetsgarden, wait for it.

We could say #monematics ...
#scicomm #sciart