| Website | https://miferg.github.io/ |
| Github | https://github.com/miferg |
| ResearchGate | https://www.researchgate.net/profile/Miguel-Romero-36 |
Some scientists have it more fun than others... ;)
Background Metagenomics is a powerful approach to study environmental and human-associated microbial communities and, in particular, the role of viruses in shaping them. Viral genomes are challenging to assemble from metagenomic samples due to their genomic diversity caused by high mutation rates. In the standard de Bruijn graph assemblers, this genomic diversity leads to complex k-mer assembly graphs with a plethora of loops and bulges that are challenging to resolve into strains or haplotypes because variants more than the k-mer size apart cannot be phased. In contrast, overlap assemblers can phase variants as long as they are covered by a single read. Results Here, we present PenguiN, a software for strain resolved assembly of viral DNA and RNA genomes and bacterial 16S rRNA from shotgun metagenomics. Its exhaustive detection of all read overlaps in linear time combined with a Bayesian model to select strain-resolved extensions allow it to assemble longer viral contigs, less fragmented genomes, and more strains than existing assembly tools, on both real and simulated datasets. We show a 3–40-fold increase in complete viral genomes and a 6-fold increase in bacterial 16S rRNA genes. Conclusion PenguiN is the first overlap-based assembler for viral genome and 16S rRNA assembly from large and complex metagenomic datasets, which we hope will facilitate studying the key roles of viruses in microbial communities. Video Abstract
#ISEPpapers 25: July, August, and September 2024 https://www.isep-protists.com/post/iseppapers-25
Your twenty-fifth digest of papers and preprints published by members of the International Society for Evolutionary Protistology is here!
JuliaR
ISEP
