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79 Posts

Bioinformatics trainers, here's the review on SAM/BAM format you wanted for your workshops :)

https://academic.oup.com/bib/advance-article/doi/10.1093/bib/bbad320/7259568?login=true

#bioinformatics #training #alignment #mapping

Sequence Alignment/Map format: a comprehensive review of approaches and applications

Abstract. The Sequence Alignment/Map (SAM) format file is the text file used to record alignment information. Alignment is the core of sequencing analysis, and

OUP Academic
the most-visited website in #bioinformatics

@jamillaaz

3. https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(23)00775-4/fulltext - plasma mcfDNA was used in monitoring the first pig-to-human heart transplant patient, detecting reactivated porcine cytomegalovirus early post-transplant, which likely contributed to the transplant rejection.

@jamillaaz
2. https://ashpublications.org/bloodadvances/article/doi/10.1182/bloodadvances.2023010208/496694/Circulating-microbial-cell-free-DNA-is-increased - plasma mcfDNA abundance changes during course of bone marrow transplant, potentially reflecting the severity of gut barrier dysfunction and health outcomes
Circulating microbial cell-free DNA is increased during neutropenia following hematopoietic stem cell transplantation

Key Points. Microbial cell-free DNA undergoes dynamic change after allo-HCTDNA derived from taxa present in the gut can be observed in circulation

American Society of Hematology

Three new papers on #mcfDNA (microbial cell free DNA) as a diagnostic tool in infectious disease and beyond!

1. https://academic.oup.com/jid/advance-article-abstract/doi/10.1093/infdis/jiad255/7219651 - sequencing of plasma mcfDNA can detect fungal infections 3 weeks before standard of care in transplant patients

#healthcare #diagnostics #microbiology #infectiousdisease #transplant #Karius

Serial quantitation of plasma microbial cell-free DNA before and after diagnosis of pulmonary invasive mold infections in hematopoietic cell transplant recipients

Plasma microbial cell-free DNA (mcfDNA) sequencing identified pathogenic molds up to three weeks before clinical diagnosis of pulmonary invasive mold infections

OUP Academic

Doing a test here to see how much reach this #mastodon account with 550 followers has compared to the same test on #Threads with a 430 Follower count.

I suspect I already know the answer.

If you see this please Boost and Like, and if you want to leave a comment feel free to that.

FINAL UPDATE - 4 Days Later

Mastodon
Likes: 3,200+
Boosts: 2,600+
Replies: 150+
Followers gained: 225

Threads
Likes: 17
Retweet/Boosts: Unknown. No stats
Replies: 4
Followers Gained: 330

Dune: Part Two | Official Trailer 2

YouTube
@jamillaaz https://github.com/smithlabcode/preseq/issues/66 Thanks to Andrew Smith for answering my question which was as idiotic as I originally thought it was.
Best practices with low-input library with high rate of PCR duplicates · Issue #66 · smithlabcode/preseq

I am trying to use Preseq to estimate the unique sequence richness using pop_size on a low input library that has been highly amplified by PCR. I am concerned that the high rate of PCR duplicates c...

GitHub
Can anyone help with a silly question - do PCR or optical duplicates need to be removed before using Preseq? I am trying to use Preseq to estimate yield on a low input library where PCR duplicates should be fairly prevalent #bioinformatics #preseq #genomics #sequencing #librarycomplexity

A draft of the human pangenome with 47 assemblies representing 13 populations https://www.nature.com/articles/s41586-023-05896-x

Interested to know more about the toolset associated with pangenome analysis 🧐

#genetics #genomics #pangenome #bioinformatics #biology