Insightful presentation by Minjia Tan at #ISCPDD. She talked about her group’s research into the use of the photoactivated ortho-nitrobenzyl alcohol (oNBA) reactive group and its application to studying protein complexes in live cells.
https://onlinelibrary.wiley.com/doi/10.1002/anie.202507254
#ChemSky #ChemBio #Electrophiles #Crosslinking #XLMS

We address these very questions using cross-linking mass spectrometry #XLMS!
https://www.biorxiv.org/content/10.1101/2022.11.16.516813v1
In XLMS, cross-linkers covalently link 2 amino acids within spatially constrained reactions. We use MS to detect the sites of these linkages (cross-links or XLs in this tweet) = low-res distance constraints representing local structure or the interface (& identity) of a PPI.
The amazing Jason Low from Sydney Uni created a absolutely vast resource of XLs for the human cell by XLing organelles with 3 different XLers - generating the largest #XLMS dataset to date for any species - almost 30,000 XLs for over 4000 proteins & 2000 PPIs!