🎉 New paper published with Prof. Pedro A. Fernandes, Dr. Ana Rita Calixto, and Roberto Pinto from Universidade do Porto!
We used QM/MM to map the catalytic mechanism of Bothrops asper sPLA2 (snake venom toxin) on the NOBA substrate.
The finding: A crucial mixed single-water/assisted water mechanism. This validates NOBA's use, giving researchers vital insights for anti-venom development! 🐍
#Snakes #SnakeVenom #Venom #BBC #TimFriede #Science #Biology #Immunity #Animals #Poison #Poisonous #Animal #Venomous #Research #antivenom
Quite an amazing story, and Tim Friede could be helping to save thousands of lives for many years to come…
'Unparalleled' snake antivenom made from man bitten 200 times https://www.bbc.com/news/articles/cr5d0l7el36o
Meet the Man who Injects Himself with Snake Venom!
https://www.youtube.com/shorts/4Xgqaidc-DU
<p>Fragments of hemorrhagic toxins that may help treat high blood pressure were found in the venom of the pit viper Cotiara, which inhabits the South of Brazil, and that of the South American bushmaster, a denizen of tropical rainforests. The molecules might one day be used in drugs with fewer adverse side effects. The researchers were particularly interested in a novel metalloproteinase-derived peptide called Lm-10a, a fragment of a hemorrhagic toxin that inhibits ACE and could potentially be used in a drug to treat blood pressure. </p> <p> </p>
A #snakevenom-analog #peptide that inhibits #SARSCoV2 and papain-like protease displays #antithrombotic activity in #mice #arterial # thrombosis model, without interfering with #bleeding time
https://thrombosisjournal.biomedcentral.com/articles/10.1186/s12959-022-00436-5
https://thrombosisjournal.biomedcentral.com/counter/pdf/10.1186/s12959-022-00436-5.pdf
Background (p-BthTX-I)2 K, a dimeric analog peptide derived from the C-terminal region of phospholipase A2-like bothropstoxin-I (p-BthTX-I), is resistant to plasma proteolysis and inhibits severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strains with weak cytotoxic effects. Complications of SARS-CoV-2 infection include vascular problems and increased risk of thrombosis; therefore, studies to identify new drugs for treating SARS-CoV-2 infections that also inhibit thrombosis and minimize the risk of bleeding are required. Objectives To determine whether (p-BthTX-I)2 K affects the hemostatic system. Methods Platelet aggregation was induced by collagen, arachidonic acid, and adenosine diphosphate (ADP) in the Chronolog Lumi-aggregometer. The coagulation activity was evaluated by determining activated partial thromboplastin clotting time (aPTT) and prothrombin time (PT) with (p-BthTX-I)2 K (5.0–434.5 µg) or 0.9% NaCl. Arterial thrombosis was induced with a 540 nm laser and 3.5–20 mg kg− 1 Rose Bengal in the carotid artery of male C57BL/6J mice using (p-BthTX-I)2 K. Bleeding time was determined in mouse tails immersed in saline at 37 °C after (p-BthTX-I)2 K (4.0 mg/kg and 8.0 mg/kg) or saline administration. Results (p-BthTX-I)2 K prolonged the aPTT and PT by blocking kallikrein and FXa-like activities. Moreover, (p-BthTX-I)2 K inhibited ADP-, collagen-, and arachidonic acid-induced platelet aggregation in a dose-dependent manner. Further, low concentrations of (p-BthTX-I)2 K extended the time to artery occlusion by the formed thrombus. However, (p-BthTX-I)2 K did not prolong the bleeding time in the mouse model of arterial thrombosis. Conclusion These results demonstrate the antithrombotic activity of the peptide (p-BthTX-I)2 K possibly by kallikrein inhibition, suggesting its strong biotechnological potential.
Scientific evidence gathered in different laboratories around the world has established that the coronavirus SARS-CoV-2 is the causative agent of COVID-19. Pregnant women are at a higher risk of developing severe COVID-19 and getting COVID-19 increases the risk of pregnancy complications, including miscarriage. Studies so far have shown that COVID-19 vaccines are highly effective at reducing the risk of severe disease and don’t increase the risk of negative pregnancy outcomes. The American College of Obstetricians and Gynecologists recommend that pregnant women get vaccinated against COVID-19.
Do COVID-19 mRNA vaccines and treatments for the virus come from snake venom and do the vaccines then cause humans to produce venom in their bodies? In addition, is nicotine the "perfect antidote" for COVID, making the vaccines unnecessary? No,...
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