Mastodawn

Development of EST-SSR Markers Based on Transcriptome Sequencing in Kodo millet (Paspalum scrobiculatum l.) - #kodomillet #genome #transcriptome #RTPCR #cDNAlibrary #ESTSSR #cDNA #Paspalumscrobiculatum #Paspalum - https://link.springer.com/article/10.3103/S0095452725060040

Development of EST-SSR Markers Based on Transcriptome Sequencing in Kodo millet (Paspalum scrobiculatum l.) - Cytology and Genetics

Abstract Kodo millet (Paspalum scrobiculatum L.) (2n = 4x = 40) mostly found in dry and semi-arid parts of Africa and Asia, also known as miraculous millets, is a staple food and a nutrient-security crop. It’s commonly known that kodo millet has the strongest tolerance to drought. Little study was done on high throughout genomic studies in relation to the creation of millets improved by genomics. In the present investigation, 21 080 571 total reads of sequence data were included in the 6.55 gigabytes of raw sequence that was produced following the genome sequencing run. After utilizing FastQC to verify the raw data quality, a total sequence of 1 087 611 and 578 123 572 bp was obtained. Three RNA samples from mature, blooming, and vegetative specimens were gathered, and the high-throughput Ion torrent sequencing method was applied to the sequencing process. In the vegetative stage, there were 1, 34, 40, and 824 raw readings; in the reproductive and mature stages, there were 1, 66, 35, 843 reads and 97, 43, 629 bp reads, respectively. At three distinct stages, the average read length was 131.6, 137.6, and 148.8 bp, in that order. The interface modules BatchPrimer3 (version 1.0) were used to construct the SSR and EST-SSR primers. Four FASTA files including contigs from the transcriptome and genome were utilized to identify SSRs. A total of thirty SSRs were found, with GC% and Tm ranges of 40–70 and 58–62°C, respectively. Of the 30 SSR and EST-SSR primers that were used for validation, only 17 were able to amplify the DNA from 27 distinct types of Kodo millet. 24 bands were obtained from the PCR amplification of 27 distinct types. We think that these findings may be applied in the future as possible candidates to enhance this significant staple crop.

SpringerLink

Lluis Montoliu Mar 14

Hace cinco años que nos confinaron en casa por una pandemia causada por un nuevo virus que podíamos detectar mediante #RTPCR, técnica que pocos entendían. Y decidí explicarla… con piezas de #TENTE … y grabé un primer video para #YouTube… y se viralizó!
https://youtu.be/CxSu61UcQXk

¿Cómo diagnosticar coronavirus con la técnica RT-PCR?

YouTube

Giuseppe Michieli Jul 11, 2024

#Multiplex Dual-Target #RTPCR for Subtyping Avian #Influenza A(#H5) Virus, Emerg Infect Dis.: https://wwwnc.cdc.gov/eid/article/30/8/24-0785_article?ACSTrackingID=USCDC_333-DM131858&ACSTrackingLabel=Latest%20Expedited%20Articles%20-%20Emerging%20Infectious%20Diseases%20Journal%20-%20July%2010%2C%202024&deliveryName=USCDC_333-DM131858

An increased #risk for #human #infection with avian influenza A(#H5N1) viruses is of concern. We developed an internally controlled, dual-target reverse transcription PCR for influenza A(H5) subtyping. This test could be used to detect influenza A(H5) in clinical samples.

Multiplex Dual-Target Reverse Transcription PCR for Subtyping Avian Influenza A(H5) Virus

PCR for Subtyping Avian Influenza A(H5) Virus

Emerging Infectious Diseases journal

micrordt Apr 20, 2024

Reverse transcription polymerase chain reaction (RT-PCR)

#Covid19, #PCR, #RNAVirus, #RTPCR, #VirusIdentification #RDT Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -

https://micrordt.wordpress.com/2024/04/20/reverse-transcription-polymerase-chain-reaction-rt-pcr/

Reverse transcription polymerase chain reaction (RT-PCR)

Medical Microbiology & RDT Labs

micrordt Apr 20, 2024

Polymerase Chain Reaction

#DNA, #DNAIsolation, #DNASequence, #PCR, #Polymerase, #RTPCR #Biotechnology, #RDT Polymerase Chain Reaction (PCR) is a widely used technique in biotechnology and molecular biology to amplify the sequence of genetic…. Medical Microbiology & Recombinant DNA Technology (RDT) Labs | Read More -

https://micrordt.wordpress.com/2024/04/20/polymerase-chain-reaction/

Polymerase Chain Reaction

Medical Microbiology & RDT Labs

Teresita Porter 🙋🏻‍♀️Dec 21, 2023

**A practical workflow for forensic species identification using direct sequencing of real-time PCR products**

https://link.springer.com/article/10.1007/s11033-023-08980-7

#qPCR #rtPCR #sequencing #DNAbarcoding #forensics

A practical workflow for forensic species identification using direct sequencing of real-time PCR products - Molecular Biology Reports

Background Forensic scientists are often required to identify species of unknown biological samples. Although methods based on sequencing of DNA barcode regions are the gold standard for species identification in single-source forensic samples, they are cumbersome to implement as routine work in forensic laboratories that perform many tests, including human DNA typing. We have developed a species identification workflow that incorporates direct sequencing with real-time PCR products (real-time PCR–direct sequencing) as the technical trick for easy testing in forensic practice. Method and results Following our workflow, DNA samples from vertebrates, such as mammals, amphibians, reptiles, birds, and fish, were subjected to species identification using vertebrate universal primers targeting each of the four DNA barcode regions. In real-time PCR melting curve analysis, humans and animals (nonhuman) could be differentiated by comparing melting temperatures, and subsequent real-time PCR–direct sequencing contributed to simplified sequencing. Searches against public DNA databases using the obtained sequences were compatible with the origin of the samples, indicating that this method might be used to identify animal species at the genus level. Furthermore, this workflow was effective in actual casework, which provided rapid test results according to the needs of the investigating agencies. Conclusions The species identification workflow will simply sequence as much as possible and can be integrated into routine forensic practice. The real-time PCR–direct sequencing used in this workflow might be beneficial not only for species identification but also for DNA sequencing by using the Sanger method for a variety of life sciences.

SpringerLink

Show thread

Anita Y Cheng Jun 20, 2023

People were more likely to get #COVID if the person they lived with had a “high viral load” (based on #rtPCR), and if they shared a bedroom with a COVID-positive person (200% more likely). Dr. Racaniello once again points out that rtPCR =/= amount of infectious virus.

#TWiVTLDR 3/

Carmela 🍉🔻Mar 17, 2023

In line with this..

PSA for Filipinos, RITM still accepts patients for RT-PCR testing. It is free for patients with symptoms and for residents in their catchment area, like Southern Manila (Muntinlupa, Parañaque, Las Piñas) and some provinces. Otherwise, there is payment of Php 2800. They also accept tests for travel. Make sure you have PhilHealth.

Not sure if they accept walk-in patients but here is the link for booking of appointments:

https://apps.ritm.gov.ph/appointment/

#Philippines #Covid19 #RTPCR

BOOM FactCheck Dec 29, 2022

The Indian Health Ministry announced that starting January 1, 2023. #RTPCR tests for passengers flying from China, Hong Kong, Japan, South Korea, Singapore, and Thailand. https://www.boomlive.in/web-stories/news/india-makes-rt-pcr-test-mandatory-for-visitors-from-5-countries-including-china-and-thailand-1343?utm_medium=Social__TW_Echobox&utm_source=Twitter#Echobox=1672314439-1

India Makes RT-PCR Test Mandatory For Visitors From 5 Countries, Including China and Thailand

The Indian Health Ministry announced that starting January 1, 2023. RT-PCR tests for passengers...

BOOM

Patrikai.com Nov 28, 2022

சீனாவிலிருந்து வருவோருக்கு கொரோனா சோதனை செய்வது குறித்து மத்தியஅரசின் அறிவுறுத்தல் பின்பற்றப்படும்! மா.சுப்பிரமணியன்
https://patrikai.com/central-government-instructions-will-follow-for-corona-test-those-coming-from-china-maa-subramanian/ via @[email protected]

#China #COVID19 #COVID #RTPCR #screening @[email protected]

சீனாவிலிருந்து வருவோருக்கு கொரோனா சோதனை செய்வது குறித்து மத்தியஅரசின் அறிவுறுத்தல் பின்பற்றப்படும்! மா.சுப்பிரமணியன்

சென்னை: சீனாவிலிருந்து வருவோருக்கு கொரோனா சோதனை செய்வது பற்றி மத்தியஅரசின் அறிவுறுத்தல் பின்பற்றப்படும் என தமிழ்நாடு மக்கள் நல்வாழ்வுத்துறை அமைச்சர் மா.சுப்பிரமணியன் கூறியுள்ளார். உலகம் முழுவதும் கொரோனா தொற்று பரவல் வெகுவாக குறைந்த நிலையில், தொற்று பரவலுக்கான காரணமான சீனவில் மீண்டும் கொரோனா அதிகரித்து வருகிறது. இதற்கிடையில், உலக சுகாதார மையமான who, விமான நிலையங்களில் ஆர்டிபிசிஆர் பரிசோதனை எடுக்க தேவையில்லை என அறிவித்து உள்ளது. ஆனால், இந்திய அரசு சீனாவில் இருந்து வரும் பயணிகளுக்கு விமான...