Creating a GPCR family activated by an inert ligand
๐งช Evolved muscarinic GPCRs, responsive only to CNO
โ๏ธ Achieved cell signaling without native ligands
๐ Used mutant receptors to study signaling pathways for msd
#GPCR #MolecularEvolution #DREADD #Pharmacology #CellEngineering
https://dev.https://tnyp.me/CcS0q4hQ/m
Directed molecular evolution of muscarinic receptors
๐ Evolved GPCRs activated by CNO, not acetylcholine
๐งช CNO activates M3 with 20-30 nM potency
๐ฌ hM4D receptor induces neuronal silencing for mtd
#GCPR #CNO #DREADD #hM3D #hM4D #molecularEvolution
https://dev.https://tnyp.me/CcS0q4hQ/m
I'm excited to present this new model to detect positive selection on regulatory sequences, which has been 3 years in the making!
Thanks to Alexandre Laverrรฉ and @Phylogenetrips for their amazing work on this project. ๐
https://doi.org/10.1101/2025.11.26.690685
Thread! #bioinformatics #molecularEvolution
๐งฉ What if the diverse functions of a single protein all trace back to one structural superfamily?
๐ Evolutionary and structural bioinformatics identifies GPR89 as a conserved member of the LIMR protein superfamily. Computational and Structural Biotechnology Journal, DOI: https://doi.org/10.1016/j.csbj.2025.11.003
๐ CSBJ: https://www.csbj.org/
#StructuralBiology #ComputationalBiology #MolecularEvolution #ProteinScience #Genomics #Proteomics #AlphaFold #MembraneProteins #Genetics #Bioinformatics
Abstract One of the most well-documented cases of successful plant invasion in Europe is the rapid spread of Himalayan balsam (Impatiens glandulifera Royle). Introduced from the Himalayas to Europe in the first half of the 19th century as an ornamental and nectar-producing plant, this species initially naturalized in the United Kingdom, from where it subsequently dispersed across the European continent. Despite the active invasion of I. glandulifera in Eastern Europe, the genetic diversity of its populations in this region has remained largely unexplored. In this study, we evaluated the variability of two chloroplast DNA (cpDNA) regions, trnS-G and rpl32-trnL(UAG), in I. glandulifera samples from Ukraine and compared the results with cpDNA variants from continental Europe, the United Kingdom, as well as from India and Pakistan. Our results reveal the widespread distribution of two distinct I. glandulifera haplotypes, T1-R1 and T2-R2, across continental Europe. These haplotypes differ in both cpDNA regions analyzed, and their divergence is inferred to have occurred within the speciesโ native range. Chloroplast DNA variation was found to be significantly higher in the native range than in the invasive range. The broad distribution of the two chloroplast haplotypes across Europe supports the hypothesis of multiple introductions of I. glandulifera into the continent. The uneven distribution of haplotypes T1-R1 and T2-R2 within Ukraine may indicate a founder effect.
Suweb Reza
Marc Robinson-Rechavi
CSBJ
Cytology and Genetics