Fabrication & validation of an affordable #OpenSource #DIY coaxial #3D #extrusion #bioprinter:

-based on #Ender3D Pro, #Python / #GCode
-2 #syringe #pump #extruders
-coaxial nozzle print head
-cost < $600

https://doi.org/10.1038/s41598-025-06478-9
#DIYbio #lab #bioprinting #instruments #hydrogel #tissue #engineering

#OpenSLS update: Generation of model #tissues with #dendritic #vascular networks via sacrificial #laser-sintered #carbohydrate templates: https://rdcu.be/b5tHy

Hardware designs: https://github.com/MillerLabFTW/OpenSLS
-R4: specialty powders for biomaterials research
-R3: nylon & wax #SLS

-#Python add-on for #Blender to generate bifurcating vascular structures
#DIYbio #OpenSource #lab #3Dprinting #bioprinter #laser #hydrogel #instruments

SciTech Chronicles. . . . . . . . .Jan 30, 2025

https://bit.ly/stc013025

#Ice Volcanism #Zavaritskii #sulfur #tephra #OSIRIS-REx #nucelobases #carbonate #phosphorus #boron #Zagros #Neotethys #Mesopotamia #Arabian #Eurasia #IO #Juno #JIRAM #gravitational #Tidal #Bioprinting #open-source #bioprinter #Printess #DIW #MM3D

#mSLAb: An #OpenSource masked #stereolithography (#mSLA) #bioprinter:

-based on Phrozen Sonic Mini 4 K
-#Arduino-temperature control & #humidification of the printing chamber

https://doi.org/10.1016/j.ohx.2024.e00543
#DIYbio #lab #instruments #hydrogel #3Dprinting #TissueEngineering

#MOS3S: #Microfluidic-assisted #OpenSource #3Dbioprinting system for the engineering of hierarchical #tissues:

-#3dprinted parts
-#syringe #pumps
-fabrication of cm scale lattice constructs

https://doi.org/10.1016/j.ohx.2024.e00527
#DIYbio #lab #instruments #hydrogel #bioprinter #tissue

3D Printing Toothpaste In the Name of Science

While we don't often see them in the hobbyist community, 3D printers that can extrude gels and viscous liquids have existed commercially for years, and are increasingly used for biological research. [Ahron Wayne] has recently been working with such a printer as part of a project to develop a printed wound dressing made of honey and blood clotting proteins, but for practice purposes, wanted to find a cheaper and more common material that had similar extrusion properties.

The material he settled on ended up being common toothpaste. In the video below you can see him loading up the cartridge of a CELLINK INKREDIBLE+ bioprinter with the minty goop, which is then extruded through a thin blunt-tip needle by compressed air. After printing out various shapes and words using the material, often times directly onto the bristles of a toothbrush, he's come up with a list of tips for printing similarly viscous substances.

First and foremost, go slow. [Ahron] says the material needs a moment to contract after being extruded if it's going to have any hope of supporting the next layer of the print. Thick layer heights are a necessity, as is avoiding sharp curves in your design. He also notes that overhangs must be avoided, and though it probably goes without saying, clarifies that an object printed from toothpaste will never be able to support anything more than its own weight.

In addition to the handful of legitimate DIY bioprinters that have graced these pages over the years, we've seen the occasional chocolate 3D printer that operated on a similar principle to produce bespoke treats, so the lessons learned by [Ahron] aren't completely lost on the hacker and maker crowd. Who knows? Perhaps you'll one day find yourself consulting this video when trying to get a modified 3D printer to lay down some soldering paste.

#3dprinterhacks #medicalhacks #bioprinter #extrusion #pasteextruder #toothpaste