Not all microscopy images turn out great. Nevertheless, they can still be cool. This one definitely has an abstract art vibe. 🖼️
#Microscopy #fluoresence #cellbiology
The image shows Zmax projection of tobacco leaf, transiently overexpressing vacuolar membrane localized AtPIEZO1 tagged with EGFP. The EGFP fluorescence is in green. The leaf was also stained, unsuccessfully, with Mitotracker, in magenta. The chlorophyll autofluorescence is blue.
When moss cells are protoplasted, they behave like spores and can rebuild the whole plant. Over four days, protoplasts, when cultured on osmotically supported media, regenerate their cell walls and differentiate into chloronema cells, which slowly start to grow and divide.
When moved to regular media, the plants start to rapidly grow and divide. Within 3 days plants expand from 1-2 cells to what you see in the image.
At one point, some chloronema cells differentiate into caulonema cells.
I love working with moss! They are even more adorable up close.
This is a single gametophore with the accompanying rhizoids from moss Physcomitrium patens. The image was made with the Olympus SZX7 dissecting scope with a DP71 camera from Olympus/Evident.
The image shows a single green gametophore from moss Physcomitrium patens. Gametophore is composed of many interlaced leaf-like structures, phyllids. Long and brownish rhizoids are protruding from the base of the gametophore.
@ehaswell