#flowcytometry is an amazing tool, but can be difficult to understand for novices. Check this out!

https://thelifesciencesmagazine.com/guide-to-understanding-flow-cytometry/

#facs #fluoresence #biotechnology #CellBiology

@biology @science @CellStemCell @biorxiv_cellbio

Can anyone describe a stable setup for a PCO camera like Edge or panda? Recommended client software? Windows version?

#fluoresence #imaging

#Mantis: #Automated high-throughput #OpenSource #microscope for #4D #imaging & analysis of the #molecular & physical architecture of #cells:

-#Python-based software included (#shrimPy, #VisCy)

https://doi.org/10.1101/2023.12.19.572435
#DIYbio #lab #instruments #LSM #fluoresence #microscopy

Thought-provoking preprint about the variable photostability of StayGold (compared to classic #GFPs) under different imaging modalities. Basically, you should a spinning disk for best results, not pointscanning confocal. Wonder if that also goes for 2-photon. The paper includes a “classification of #fluoresence #microscopy” according to ISO 10934:2020 !!!

https://www.biorxiv.org/content/10.1101/2023.11.23.568490v1.full.pdf

Not all microscopy images turn out great. Nevertheless, they can still be cool. This one definitely has an abstract art vibe. 🖼️
#Microscopy #fluoresence #cellbiology

The image shows Zmax projection of tobacco leaf, transiently overexpressing vacuolar membrane localized AtPIEZO1 tagged with EGFP. The EGFP fluorescence is in green. The leaf was also stained, unsuccessfully, with Mitotracker, in magenta. The chlorophyll autofluorescence is blue.