| The biology we try to understand | https://doi.org/10.1038/s41389-019-0136-0 |
| The biophysics we try to understand | https://doi.org/10.1016/j.isci.2019.04.021 |
Barbara
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- 55 Following
- 18 Posts
Misplaced protein phys-chemist playing with biology. Dog lover. Landed in Yorkshire via Rome, Brighton, Nottingham, Chester. She/her. Everything I blurb, it's my own opinion.
@steveroyle terribly sad
Stephen RoyleDeeply saddened by the news that my friend David Stephens died last night.
David was an outstanding cell biologist based at Bristol whose work spanned #MembraneTraffic #cytoskeleton #ciliogenesis and more. He was “one of the good guys”. Kind, supportive, generous with his time, and rigorous about his science. I’ll miss him.
...and thank you to the Royal Society for a Short Industry Fellowship! Looking forward to work with my colleagues at Astrazeneca in Cambridge👌
@steveroyle 😃my story with ESCRT-III it’s longer than you can imagine. This is work of extremely smart students and truly great collaborations. Maybe there is another little story to come out. Who knows? I write slowly. Looking forward to reading more of your membrane discoveries!
Simone CoppolaI made this account ages ago but never really used it, so I guess it's time for an #introduction !
I am a postdoctoral researcher at the University of Warwick, where I study how bacteria divide! I'm a big fan of super-resolution microscopy techniques🔬, and spend my days balancing wetlab work, microscope building and developing image analysis tools!
One half of a microscopy loving duo with @MAFesenko 🥰
Big thanks to @quantixed for the list of accounts to follow: my feed looks way better now!
So proud of this 2019 work 😀. It was the effort of 3 PhD students, international collaborators and friends (& ~£0!). Still doing well for citations notwithstanding follow up work on 'bigger' journals out there. https://www.nature.com/articles/s41389-019-0136-0
ESCRT-III is necessary for the integrity of the nuclear envelope in micronuclei but is aberrant at ruptured micronuclear envelopes generating damage - Oncogenesis
Micronuclei represent the cellular attempt to compartmentalize DNA to maintain genomic integrity threatened by mitotic errors and genotoxic events. Some micronuclei show aberrant nuclear envelopes (NEs) that collapse, generating damaged DNA that can promote complex genome alterations. However, ruptured micronuclei also provide a pool of cytosolic DNA that can stimulate antitumor immunity, revealing the complexity of micronuclear impact on tumor progression. The ESCRT-III (Endosomal Sorting Complex Required for Transport-III) complex ensures NE reseals during late mitosis and is repaired in interphase. Therefore, ESCRT-III activity maybe crucial for maintaining the integrity of other genomic structures enclosed by a NE. ESCRT-III activity at the NE is coordinated by the subunit CHMP7. We show that CHMP7 and ESCRT-III protect against the genomic instability associated with micronuclei formation. Loss of ESCRT-III activity increases the population of micronuclei with ruptured NEs, revealing that its NE repair activity is also necessary to maintain micronuclei integrity. Surprisingly, aberrant accumulation of ESCRT-III are found at the envelope of most acentric collapsed micronuclei, suggesting that ESCRT-III is not recycled efficiently from these structures. Moreover, CHMP7 depletion relieves micronuclei from the aberrant accumulations of ESCRT-III. CHMP7-depleted cells display a reduction in micronuclei containing the DNA damage marker RPA and a sensor of cytosolic DNA. Thus, ESCRT-III activity appears to protect from the consequence of genomic instability in a dichotomous fashion: ESCRT-III membrane repair activity prevents the occurrence of micronuclei with weak envelopes, but the aberrant accumulation of ESCRT-III on a subset of micronuclei appears to exacerbate DNA damage and sustain proinflammatory pathways.
@steveroyle virtual meetings are good too. Life just gets too complicated sometimes to have time to go somewhere.