This work would not have been possible without the commitment, work, and enthusiasm of a truly diverse team starting from Ryder Gwinn, Rebeca de Frates, Anirban Nandi, and Rusty Mann.

Importantly, we found that the three conductances shaping #singlecell responses also drove higher #excitability in a #human-like #brain-circuit, as observed in human patients with late-stage #temporal #lobe #epilepsy.

We used #parallelcomputing and #biophysical #simulations to go from deep, #multimodal #feature changes to #causal #differences. Result? Three conductances explained #phenotypic, #disease-related changes, an observation supported by subsequent #snRNA analysis.

Long story short: we found that disease progression gave rise to changes across all #modalities. What are the #underlying #causes of these cellular phenotypic changes and how are they manifested at the #brain #circuit-level, for example in the form of #seizures?

We decided to focus our study on a single #celltype, dentate gyrus granule cells, and produce multiple #singlecell #data #modalities: #snRNA, #ephys, reconstructed morphologies and synaptic density.

Back in 2016, together with Jonathan Ting we thought looking at #singlecells in the living, resected hippocampal tissue excised from #epilepsy #patients was such a unique opportunity to study the #disease and, ultimately, make a #difference in the lives of #patients.