A tripartite cell-free translation system to study mammalian translation.
#RNA #Translation #Cell-FreeSystem #Mammals #Protocols
This protocol presents a tripartite mammalian cell-free translation system, comprising cytoplasmic and ribosome fractions combined with endogenous or synthetic mRNAs, permitting flexible reconstitution and careful dissection of the mechanisms of translation.
A high-performance IoT development platform built with Rust, designed for multi-protocol support and real-time data processing. This platform supports MQTT, WebSockets (WS), TCP, and CoAP protocols...
Web 3.0 Requires Data Integrity
If you’ve ever taken a computer security class, you’ve probably learned about the three legs of computer security—confidentiality, integrity, and availability—known as the CIA ... https://www.schneier.com/blog/archives/2025/04/web-3-0-requires-data-integrity.html
If you’ve ever taken a computer security class, you’ve probably learned about the three legs of computer security—confidentiality, integrity, and availability—known as the CIA triad. When we talk about a system being secure, that’s what we’re referring to. All are important, but to different degrees in different contexts. In a world populated by artificial intelligence (AI) systems and artificial intelligent agents, integrity will be paramount. What is data integrity? It’s ensuring that no one can modify data—that’s the security angle—but it’s much more than that. It encompasses accuracy, completeness, and quality of data—all over both time and space. It’s preventing accidental data loss; the “undo” button is a primitive integrity measure. It’s also making sure that data is accurate when it’s collected—that it comes from a trustworthy source, that nothing important is missing, and that it doesn’t change as it moves from format to format. The ability to restart your computer is another integrity measure...
Бинарный формат вместо текста
Как быстро, без боли и страданий организовать хранение структурированных данных в бинарном формате. А затем и их передачу при необходимости. А потом, немного подумав, ещё их обнаружение в «замусоренном» потоке.
https://habr.com/ru/articles/895902/
#rust #protocols #parsing #binary #communication #no_database #storage #binary_storage #filtering
The inspiration for above mentioned re-reading of #RFC9413 for fun.
I've always respected Jon Postel's work and, from what I know, took them as inspiration and "guiding principles" in my work as a network engineer and architect. It's good to see, in my opinion, them getting re-evaluated rather than thrown out with the bathwater. YMMV of course.
https://alexgaynor.net/2025/mar/25/postels-law-and-the-three-ring-circus/
As good a time as any to re-read an #RFC and re-visit Postel's "law" - "be conservative in what you send, and liberal in what you accept".
I appreciate the consideration put into this as well as the pertinent examples.
https://datatracker.ietf.org/doc/html/rfc9413
#protocols #standards #networking
The main goal of the networking standards process is to enable the long-term interoperability of protocols. This document describes active protocol maintenance, a means to accomplish that goal. By evolving specifications and implementations, it is possible to reduce ambiguity over time and create a healthy ecosystem. The robustness principle, often phrased as "be conservative in what you send, and liberal in what you accept", has long guided the design and implementation of Internet protocols. However, it has been interpreted in a variety of ways. While some interpretations help ensure the health of the Internet, others can negatively affect interoperability over time. When a protocol is actively maintained, protocol designers and implementers can avoid these pitfalls.
<div xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" class="section"> <a class="named-anchor" id="d13359648e562"> <!-- named anchor --> </a> <h5 class="section-title" id="d13359648e563">Background</h5> <p dir="auto" id="d13359648e565">In the United States, the landscape of unpaid care delivery is both challenging and complex, with millions of individuals undertaking the vital role of helping families (broadly defined) manage their health care and well-being. This includes 48 million caregivers of adults, 42 million of whom are caregivers of adults aged 50 years or older. These family care partners provide critical and often daily support for tasks such as dressing and bathing, as well as managing medications, medical equipment, appointments, and follow-up care plans. </p> </div><div xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" class="section"> <a class="named-anchor" id="d13359648e567"> <!-- named anchor --> </a> <h5 class="section-title" id="d13359648e568">Objective</h5> <p dir="auto" id="d13359648e570">This study aimed to implement a novel patient portal–based intervention to identify, engage, and support care partners in clinical settings. </p> </div><div xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" class="section"> <a class="named-anchor" id="d13359648e572"> <!-- named anchor --> </a> <h5 class="section-title" id="d13359648e573">Methods</h5> <p dir="auto" id="d13359648e575">The project team collaborated with 3 health care organizations (6 primary care practices in total) to design and implement a patient portal–based intervention. Three days in advance of a visit, patients were invited to log on to their patient portal account and answer a brief questionnaire as part of the routine electronic check-in process asking them to (1) identify themselves as the patient or someone answering for the patient, (2) report major life changes, (3) set the agenda for the upcoming visit, and (4) report on care partner responsibilities. Respondents’ answers to this brief questionnaire were available to providers ahead of the visit. Patients with care partner responsibilities, as well as care partners answering the questionnaire on behalf of patients, were provided a link to the ARCHANGELS Caregiver Intensity Index to measure the intensity of their caregiving role and motivate care partners to connect with suggested state and local resources. </p> </div><div xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" class="section"> <a class="named-anchor" id="d13359648e577"> <!-- named anchor --> </a> <h5 class="section-title" id="d13359648e578">Results</h5> <p dir="auto" id="d13359648e580">The intervention was launched in September 2022 at Organization A. Organization B launched in May 2023 in one clinic and June 2023 in the other. In focus groups, staff and clinicians reported that the intervention was easy to implement and did not cause workflow disruption. At 6 months post implementation, across both organizations, a total of 22,152 patients had received questionnaires and 13,825 (62.4%) had submitted completed questionnaires. Full data will be reported at the completion of the intervention period. </p> </div><div xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" class="section"> <a class="named-anchor" id="d13359648e582"> <!-- named anchor --> </a> <h5 class="section-title" id="d13359648e583">Conclusions</h5> <p dir="auto" id="d13359648e585">Early results suggest that the intervention could be an easily scalable and adaptable method of identifying and supporting care partners in clinical settings. </p> </div><div xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" class="section"> <a class="named-anchor" id="d13359648e587"> <!-- named anchor --> </a> <h5 class="section-title" id="d13359648e588">International Registered Report Identifier (IRRID)</h5> <p dir="auto" id="d13359648e590">DERR1-10.2196/66708</p> </div>
A great video about why #Bluesky is NOT recommended as a truly open and independent alternative to mainstream #socialmedia.
@niccolove @niccolo_ve
#activitypub #fediverse #atproto #protocols #thefutureisfederated
Amelia Cervera 🧬
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Carla
Kevin Bowen 
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Tiziano 