Whole-mouse imaging the hard way: Clearing, then slicing + block-face imaging with beam-scan / sensor scan-line synchronization and continuous stage movement in an oblique imaging setup (ViSOR).
This time, they scaled it up to whole-mouse and used pre-cleared mouse bodies (ARCHmap-blockface-ViSOR):

High-speed mapping of whole-mouse peripheral nerves at subcellular resolution
Shi et al., preprint at biorxiv 2025
https://doi.org/10.1101/2025.01.22.632569

#neuroscience #lightsheet #microscopy #MicroscopyMonday

Here's a #MicroscopyMonday segmentation of some neurons from the optic lobe, or visual processing area of the #Drosophila fruit fly's brain. The #connectome for the full optic lobe was released in 2024 by #HHMIJanelia, Google, and the University of Cambridge, with support from the Wellcome Trust.
This segmentation of #MicroscopyMonday data reconstructs neurons of the "MANC", or male adult nerve cord, from the #Drosophila fruit fly. The nerve cord is like a spinal cord and connects the brain and muscles. #HHMIJanelia, Google, and the University of Cambridge released this work in 2023.
A use of #MicroscopyMonday data is making segmentations. Here's one, of #Drosophila protocerebral bridge and nodulus neurons, from the 2020 Hemibrain by #HHMIJanelia and Google. MichaΕ‚ Januszewski and Viren Jain's team made an initial segmentation with #AI flood-fill algorithms...

Ya boi is back with more microscope photos :3

Here are a few pics of a cool looking filamentous algae I found looking at more bog water under the microscope. I don't know too much about algae species (yet!) so I can't say much in terms of identification for this specimen, but it's certainly pretty to look at!

#microscopy #microscopymonday #algae (does this really count as #NaturePhotography)

#MicroscopyMonday – No this is NOT an aerial view of the Grand Canyon.

This is a scanning electron microscopy image of the cranial sutures in the rat skull. πŸ”¬πŸ’€πŸ€ These are fibrous joints where the bones of the skull interdigitate, allowing for flexibility and growth during infancy and early childhood before fusing in adulthood.

πŸ”¬πŸ¦΄πŸ§ͺ

#bone #electronmicroscopy #imaging #biomaterials #science #medicine #biology #development #osteology #evolution

Happy #MicroscopyMonday!
Here is a kymograph of a cell crawling into a microchannel. Each horizontal row of pixels represents a line drawn from outside (green) through a channel (purple) at 5 second timepoints. ~1/2 way down (20 min) a cell fully enters the channel and takes off!

Happy #MicroscopyMonday! Here are somme amoebae crawling into channels

LUT: JDM_Phase Ink Mint-Cherry

🌟 Pre-vacation #MicroscopyMonday magic rendered by @Daniel_Stabley! 🌟 This is a showstopper: our top plasma membrane probes light up neurons and glia. But wait, there's more! The grand finale? A lattice light sheet stunner showing off its isotropic prowess in the XZ view. Confocal 3D rotations, eat your heart out! πŸ§ πŸ”¬βœ¨ #Neuroscience #MicroscopyMagic #NextLevelImaging #cellbio #cellfie #scicomm #neuroscience #Microscopy
⚠️ That's what a leaky blood vessel looks like in the microscope. Astrocytes (🟑Gfap) around the blood vessel become reactive, inducing neuroinflammatory responses and attacking the surrounding neurons (πŸ”΅Neun) in the pathway. #MicroscopyMonday
#LongCovid