Ich bin voll der Fan von #StachelUndHerz . ( ein #Podcast )
Und finde bisschen traurig, dass sie sich offensichtlich weiterhin so abhäning von #Instagram usw. #BigTech fühlen :-( .
"folgt uns, gebt uns ein Like, kommentiert auf allen Plattformen, das hilft uns " .
Dabei gibt die #vem doch Sicherheit. Und #Relevanz sollte doch auch anders entstehen können, oder schon da sein.
@innuendo @jernej__s @ZachWeinersmith
Our eFIB-SEM (as per Xu et al. 2017 https://elifesciences.org/articles/25916 ) runs 24/7 for up to 8 weeks, when it runs out of source. And it's essential that it does so, uninterrupted, since it's imaging at nanometre resolution, milling 2 nm slices at a time with an ion beam and then repeatedly imaging the block face. The process is unsurprisingly very sensitive; was hard enough to overcome the reheat cycle of the instrument itself (every 60 hours or so) by detecting it, de-engaging the beams and then seamlessly re-engaging with the block face.
A brief video of the kind of data we acquire:
https://youtu.be/p4MH4-I-P5I
The MS Windows computer deciding to reboot on its own is unacceptable.
#ElectronMicroscopy #Zeiss #FIBSEM #VolumeEM #vEM #Drosophila
Nuno da Costa at the Allen Institute for Brain Research is hiring:
"We are seeking an exceptional individual to develop innovative sectioning techniques for a large scale Electron Microscopy pipeline."
Scientist I Connectomics Allen Institute for Brain Science The mission of the Allen Institute is to understand the principles that govern life and to advance health Our creative and multi dimensional teams focus on answering some of the biggest questions in bioscience We accelerate foundational research catalyze bold ideas develop tools and models and openly share our science to make a broad transformational impact on the world The mission of the Allen Institute for Brain Science is to accelerate the understanding of how the human brain works in health and disease Using a big science approach we generate useful public resources drive technological and analytical advances and discover fundamental brain properties Built on the foundational knowledge of brain cell types the mission of the Allen Institute for Brain Science is to conduct a series of large scale projects designed to create CAP Complete Accurate Permanent datasets and experimental tools; gain fundamental knowledge in the development evolution connectivity and function of brain cell types; move towards a better understanding of mammalian brain function in healthy and diseased conditions; and disseminate the data knowledge and tools broadly with the community to accelerate research across the neuroscience field We are seeking an exceptional individual to develop innovative sectioning techniques for a large scale Electron Microscopy pipeline The scientist will join a team responsible for scaling and automating the cutting of whole mouse brain samples prepared for Electron Microscopy This will involve developing and optimizing robotic components to carefully control a nanometer scale mechanical process over centimeter scale ultrathin sections across thousands of iterations The goal of the research is to create technologies that allow the complete mapping of synaptic connectivity of individual neurons across the entire mouse brain something that has never been done before The scientist will be an essential member of the Connectomics department studying cell types and will also have opportunities for data analysis in later phases of the project The team already includes several Electron Microscopy experts and therefore welcomes applicants that are new to electron microscopy but have a strong interest in automation and in the overall goals of the project Examples of our previous work related to this position can be found on httpswwwnaturecomcollectionsbdigiaicbdhttpswwwnaturecomarticless41467 020 18659 3httpselifesciencesorgarticles76534httpswwwcellcomcellpdfS0092 86742200134 9pdfhttpswwwpnasorgdoi101073pnas2202580119At the Allen Institute we believe that science is for everyone and should be open to everyone We are dedicated to combating biases and reducing barriers to STEM careers more broadly We also believe that science is better when it includes different perspectives and voices We strive to make the Allen Institute a place where everyone feels like they belong and are empowered to do their best work in a supportive environment We are an equal opportunity employer and strongly encourage people from all backgrounds to apply for our open positions Essential Functions Collaborate with others to further scale cutting whole mouse brain samples for electron microscopyAutomate ultramicrotome cutting serial sections for volumetric Electron MicroscopyRobotic manipulation for section delivery with high precision and minimal section lossPublishpresent findings in peer reviewed journalsscientific conferencesNote Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions This description reflects managements assignment of essential functions; it does not proscribe or restrict the tasks that may be assigned Required Education and Experience PhD in Neuroscience Computer Science Engineering or equivalent combination of degree and experienceStrong background in automation or roboticsStrong programming skills please share GitHub repository as part of job applicationExperience with software engineering best practices eg version control and code reviewsPreferred Education and Experience Experience in ConnectomicsStrong publication recordAbility to work independently and within a collaborative multi disciplinary environment to meet aggressive timelinesExceptional oral and written communication and organizational skillsAbility to guide teams and coordinate activities within and across institutions including coachingmentoring junior membersWork Environment Laboratory environment often entering labs where experiments take place Laboratory environment possible chemical biological or other hazardous substances exposureThis may include wearing personnel protective equipment PPEWorking at a desk with computer analyzing dataPhysical Demands Fine motor movements in fingershands to operate computers and other office equipment; repetitive motion with lab equipmentPosition TypeExpected Hours of Work This role is currently required to work onsite We are a Washington State employer and the primary work location for all Allen Institute employees is 615 Westlake Ave N; any remote work must be performed in Washington StateTravel Occasional travel to collaborator sites Occasional attendance and participation in national and international conferences may be required Additional Comments Please note this opportunity offers relocation assistancePlease note this opportunity may offer visa sponsorshipAnnualized Salary Range 86150 106650 Final salary depends on the required education for the role experience level of skills relevant to the role and work location where applicable Benefits Employees and their families are eligible to enroll in benefits per eligibility rules outlined in the Allen Institutes Benefits Guide These benefits include medical dental vision and basic life insurance Employees are also eligible to enroll in the Allen Institutes 401k plan Paid time off is also available as outlined in the Allen Institutes Benefits Guide Details on the Allen Institutes benefits offering are located at the following link to the Benefits Guide httpsalleninstituteorgcareersbenefits It is the policy of the Allen Institute to provide equal employment opportunity EEO to all persons regardless of age color national origin citizenship status physical or mental disability race religion creed gender sex sexual orientation gender identity andor expression genetic information marital status status with regard to public assistance veteran status or any other characteristic protected by federal state or local law In addition the Allen Institute will provide reasonable accommodations for qualified individuals with disabilities
"SmartEM: machine learning-guided electron microscopy", by Meirovitch et al. 2025 (Shavit's, Samuel's and Lichtmann's lab).
SmartEM is a ‘smart’ pipeline for electron microscopy-based data acquisition for connectomics. In order to efficiently image large datasets, the approach involves imaging at short pixel dwell times and identifying problematic regions that are then imaged with longer dwell times and therefore higher quality.
UC Berkeley's electron microscopy lab uploaded some older videos and presentations, very interesting to watch!
https://www.youtube.com/@UCBEML
I liked this physical stack of images of John Kinnamon's presentation on serial section TEM (https://www.youtube.com/watch?v=uZsRGweYk7c&t=1408s), compared to our modern image stack in computer memory.
#Jesus #vem #para #buscar a #igreja #dessa #terra
via YouTube https://www.youtube.com/watch?v=H28heMUswkY
Happy to be involved in UNICIL, our new Wellcome Trust Discovery project coordinated by @micromotility.bsky.social
We will study ciliary dynamics across scales and organisms. A long-term #postdoc position will be available at Heidelberg University @uniheidelberg shortly.
@uniofexeter press release:
Joachim (he/him, white)
Kpop in Japan
Albert Cardona
RFT HiFi
Lukas Grünewald
fps3000
Gaspar Jekely