yellow β€” #tetrad thread
yellow β€” #tetrad thread
yellow β€” #tetrad thread
yellow β€” #tetrad thread

#TetradTuesday

Did some tetrad dissection of fission yeast (S. pombe) spores on Friday 😊
Today, they are showing pretty colonies πŸ₯³

Tetrad = four spores produced after yeast meiosis

#TetradDissection #Tetrad #Spore #Yeast #FissionYeast #Science #Pombe

Did some yeast (S. pombe) tetrad dissection πŸ˜‰
It’s pretty meditating on Friday night after Happy Hour ☺️
Hoping to get some nice results 😊

#Yeast #Pombe #Tetrad #Science #Genetics #Meditation #HappyHour

@AS_Katoch

#Lotus life-cycle
#Tetractys-structure and
#Pythagorean logic of
#monad (Unity - U),
#dyad (Power - P),
#triad (Harmony - H),
#tetrad (Cosmos - C):

01 Seed: U
02 Germination: P
03 Roots: H
04 Stem: C
05 Leaf: H
06 Bud: P
07 Blossoming: U
08 Full Flower: C
09 Wilt: H
10 Seed Pod: P

Stumbling again across 2013 paper doing #yeast #tetrad lineage tracing https://www.nature.com/articles/nmeth.2479
They do this by transforming in barcoded plasmids before sporulation, but then you need to pick out clones and sequence each well

Thinking an elaboration on this could use the interactionSeq system/collection from Sasha Levy.... 1/

High-throughput tetrad analysis - Nature Methods

Barcode-enabled sequencing of tetrads (BEST) allows isolation and genotyping of individual yeast spores while maintaining their tetrad relationships.

Nature

β€œWe are the new media”

#COSMOS :
retrieves the blogosphere;
obsoleces 'social media';
enhances (e)quality;
downregulates the terror.

#tetrad #mcluhan #cafe