PLOS BiologyJan 6, 2025
Nitrogen-starvation of #FissionYeast inactivates growth pathways (incl TORC1) & can trigger sexual reproduction. @SophieMartinLab use proteomic & phosphoproteomic changes during sexual development to show that TORC1 is reactivated by pheromone signaling #PLOSBiology https://plos.io/4h3OUVO
Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast

Fission yeast cells reproduce sexually when starved for nitrogen, a condition that leads to the inactivation of growth pathways such as TORC1. This study generates proteomic and phosphoproteomic datasets to describe the signaling changes that occur during sexual development, revealing that TORC1 is reactivated by pheromone signaling.

Eishi NoguchiNov 22, 2024

Belated introduction after more than two years on Mastodon 😊
While I post a lot about #Nature, #River, #FlyFishing and #IPA, I use the fission yeast Schizosaccharomyces pombe for my research. At some point, I plan to brew #Beer🍺

#FissionYeast #Pombe #Science #SchizosaccharomycesPombe

PLOS BiologyNov 8, 2024
#FissionYeast canonically need #microtubules & Tea1-Tea4 complex to establish new growth poles. @Ptcgar &co identify a parallel pathway involving actin & Rgf1-Rho1 that marks the growth poles independently of Tea1-Tea4 #PLOSBiology https://plos.io/3CgOZ9f
Rho1 and Rgf1 establish a new actin-dependent signal to determine growth poles in yeast independently of microtubules and the Tea1–Tea4 complex

The establishment of new growth poles in fission yeast canonically depends on microtubules and the Tea1-Tea4 complex. This study identifies an alternative pathway dependent on actin and Rgf1-Rho1 that acts in parallel and marks the growth poles independently of Tea1-Tea4.

Eishi NoguchiNov 1, 2024

Got some pink yeast 😊

Can we make pink beer if we use this yeast strain ??? πŸ€”

The ade6 gene mutation causing red pigments for this color change in the fission yeast S. pombe πŸ˜‰

#Pombe #FissionYeast #Yeast #Science #Beer #Pink

Eishi NoguchiOct 29, 2024

#TetradTuesday

Did some tetrad dissection of fission yeast (S. pombe) spores on Friday 😊
Today, they are showing pretty colonies πŸ₯³

Tetrad = four spores produced after yeast meiosis

#TetradDissection #Tetrad #Spore #Yeast #FissionYeast #Science #Pombe

PLOS BiologyNov 9, 2023
Selective macroautophagy of the #EndoplasmicReticulum (ER) and the #nucleus, known as ER-phagy and nucleophagy respectively, are poorly understood. A study in #FissionYeast reveals that conserved ER-shaping protein Yep1 (REEP1-4 ortholog) is required for these processes; in its absence, #ERphagy/#nucleophagy cargo structures accumulate in the cytoplasm #PLOSBiology https://plos.io/464ZfuI
The ortholog of human REEP1-4 is required for autophagosomal enclosure of ER-phagy/nucleophagy cargos in fission yeast

Selective macroautophagy of the endoplasmic reticulum (ER) and the nucleus, known as ER-phagy and nucleophagy respectively, are poorly understood. This study in fission yeast reveals that a conserved ER-shaping protein is required for these processes; in its absence, ER-phagy/nucleophagy cargo structures (not enclosed within autophagosomes) accumulate in the cytoplasm.

The EMBO JournalMar 23, 2023

How is #biomass production controlled during cell growth and the cell cycle?

New assay by Clovis Basier & Paul Nurse @thecrick using unperturbed exponentially growing populations shows how #cellcycle and cell size affect global rates of RNA and protein synthesis in #fissionyeast

#RefereedPreprint c/o @ReviewCommons now published in revised form!

https://www.embopress.org/doi/10.15252/embj.2022113333