The EMBO JournalMay 21, 2024

#Optogenetic manipulation of focal adhesion targeting shows that local #actomyosin traction force development induces focal #adhesion sliding and disassembly

Julien Aureille, Alexander Bershadsky et al

https://www.embopress.org/doi/full/10.1038/s44318-024-00114-4

PLOS BiologyApr 24, 2024
#Lysosomes are crucial cellular degradation centers, but how can one selectively modulate their activity? This study describes novel #optogenetic tools for the light-inducible manipulation of lysosomal physiology in cells & in #Celegans #PLOSBiology https://plos.io/49WGds5
Optogenetic manipulation of lysosomal physiology and autophagy-dependent clearance of amyloid beta

Lysosomes are cellular degradation centers, critical for maintaining homeostasis. Selective modulation of lysosomal activity has remained a challenge to date; this study reports the development of lysosome-targeted optogenetic tools for the light-inducible manipulation of lysosomal physiology in cells and in C. elegans.

CellBioNewsMar 11, 2024

Research team develops new technique to release and study individual #proteins in #cells.

#microscopy #optogenetic

https://phys.org/news/2024-03-team-technique-individual-proteins-cells.html

Research team develops new technique to release and study individual proteins in cells

A research team led by biochemist Professor Helge Ewers from Freie Universität Berlin has developed a new technique for the light-mediated release and investigation of proteins in live cells. The technique makes use of a laser pulse to control the release of tagged protein molecules within a cell, allowing for the molecules' function to be more clearly observed. The team believes that this method has a wide variety of potential applications in future scientific research.

Phys.org
Bose-Einstein-KondensatFeb 23, 2024

#LITOS: A versatile #OpenSource #LED #illumination tool for #optogenetic stimulation:

-32 × 64 LED matrix
-#ESP32-controlled via #WLAN
-usable with different formats of multi-well plates, petri dishes & flasks.

https://doi.org/10.1038/s41598-022-17312-x
#DIYbio #lab #instruments #optogenetics

bkJan 12, 2024
#Optogenetic stimulation of GABAergic projections from the Ventral Pallidum (VP) to the Ventral Tegmental Area (VTA) enhances reward reinforcement but not seeking in rats (#Neuroscience , #AffectiveNeuroscience )...
https://www.cell.com/cell-reports/pdf/S2211-1247(23)01680-7.pdf
eLifeDec 30, 2023
Opto-RhoGEFs is an #optogenetic toolbox that can help researchers visualise and manipulate the barrier between endothelial cells like the ones in this clip. #12DaysOfeLife #Optogenetics https://elifesciences.org/articles/84364?utm_source=mastodon&utm_medium=social&utm_campaign=organic_12Days
Opto-RhoGEFs, an optimized optogenetic toolbox to reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength

Optogenetic activation of Rho GTPases provides spatiotemporal control over endothelial cell shape and enables control over endothelial barrier function by manipulating the cell-cell overlap with blue light.

eLife
Scientific FrontlineDec 15, 2023
Using a sophisticated #optogenetic approach, the researchers have discovered a previously unknown #endogenous acid sensor in #plant #cells. And they have discovered in the guard cells of leaves that there is a #calcium store that plays an important role in processing #proton signals in #cellular responses.
#Biology #sflorg
https://www.sflorg.com/2023/12/bio12152301.html
Acid Sensor and Calcium Store Discovered in Plants

When plants are infected by pathogens, suffer from a lack of water or have to react to other external stimuli

Fabrizio MusacchioDec 10, 2023

New work by Sanzeni et al. (2023): "Mechanisms underlying reshuffling of #visual responses by #optogenetic stimulation in mice and monkeys"

🌍 https://www.cell.com/neuron/fulltext/S0896-6273(23)00706-7

#Neuroscience #VisualCortex #Modelling

Bose-Einstein-KondensatNov 15, 2023

#OptoPi: An #OpenSource #3Dprinted flexible platform for the analysis of small #animal #behaviour:

-customisable #VIS/#IR #illumination, allows for #optogenetic stimulation
-#imaging by #RaspberryPi #camera
-#Arduino control

https://doi.org/10.1016/j.ohx.2023.e00443
#DIYbio #lab #instruments

eLifeOct 1, 2023
August's most-read Tools paper presents an #optogenetic toolbox to reversibly control Rho #GTPase activity for manipulating the barrier between #endothelial cells: https://elifesciences.org/articles/84364?utm_source=mastodon&utm_medium=social&utm_campaign=submissions_organic
Opto-RhoGEFs, an optimized optogenetic toolbox to reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength

Optogenetic activation of Rho GTPases provides spatiotemporal control over endothelial cell shape and enables control over endothelial barrier function by manipulating the cell-cell overlap with blue light.

eLife